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首页> 外文期刊>Infection and immunity >Immunization against chlamydial genital infection in guinea pigs with UV-inactivated and viable chlamydiae administered by different routes.
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Immunization against chlamydial genital infection in guinea pigs with UV-inactivated and viable chlamydiae administered by different routes.

机译：通过不同途径施用的灭活紫外线的活衣原体衣原体免疫豚鼠衣原体生殖器感染。

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Female guinea pigs were immunized with viable or UV light-inactivated chlamydiae (agent of guinea pig inclusion conjunctivitis), belonging to the species Chlamydia psittaci, by intravenous, subcutaneous, oral, or ocular routes. All animals were then inoculated vaginally with viable chlamydiae to determine the extent of protection against challenge infection induced by the various regimens. The course of genital infection was significantly reduced in intensity in all groups of animals except the unimmunized controls and those animals immunized orally with inactivated antigen. Guinea pigs immunized with viable antigen were more likely to develop resistance to challenge infection and, in general, had a significantly greater degree of protection than animals immunized with inactivated antigen. No one route seemed superior in producing a protective response. Animals in all groups demonstrating protection developed serum and secretion immunoglobulin G antibody responses to chlamydiae. Lymphocyte proliferative reactions to chlamydial antigen were variable among groups. Immunoblot analysis of serum and secretions indicated a wide range of antibody specificities, but most protected animals produced antibodies to the major outer membrane protein, lipopolysaccharide, and the 61-kilodalton protein. No definitive associations could be made between the increased ability of immunization with viable organisms to produce resistance to challenge infection and a particular immune parameter. These data indicate that viable chlamydiae given by various routes are able to induce a strong immune response which can provide resistance against reinfection in some cases or at least reduce the degree of infection to a greater degree than inactivated antigen. However, complete resistance to genital tract infection may be difficult to obtain and alternate immunizations strategies may have to be developed.
机译：用活的或紫外线灭活的衣原体（豚鼠包膜结膜炎的试剂）衣原体衣原体物种对雌性豚鼠进行静脉内，皮下，口服或眼部免疫。然后将所有动物用活衣原体阴道接种阴道，以确定针对各种方案所引起的攻击性感染的保护程度。除未免疫对照和经灭活抗原口服免疫的所有动物外，所有组动物的生殖器感染过程强度均明显降低。用活抗原免疫的豚鼠比用灭活抗原免疫的动物更有可能对攻击感染产生抵抗力，并且通常具有明显更高的保护程度。在产生保护反应方面似乎没有一条途径是优越的。证明具有保护作用的所有组的动物均产生了针对衣原体的血清和分泌性免疫球蛋白G抗体应答。各组间对衣原体抗原的淋巴细胞增殖反应不同。血清和分泌物的免疫印迹分析表明，抗体的特异性范围很广，但是大多数受保护的动物对主要的外膜蛋白，脂多糖和61-千达尔顿蛋白产生抗体。在有活力的生物体产生增强的抗攻击性感染的免疫能力与特定的免疫参数之间没有明确的关联。这些数据表明，通过各种途径给予的活衣原体能够诱导强免疫应答，这在某些情况下可以提供对再感染的抵抗力，或者至少比灭活抗原降低感染程度。但是，可能难以获得对生殖道感染的完全抵抗力，因此可能必须制定其他免疫策略。

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