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首页> 外文期刊>Infection and immunity >Activation of human monocytes by Streptococcus mutans serotype f polysaccharide: immunoglobulin G Fc receptor expression and tumor necrosis factor and interleukin-1 production.
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Activation of human monocytes by Streptococcus mutans serotype f polysaccharide: immunoglobulin G Fc receptor expression and tumor necrosis factor and interleukin-1 production.

机译:变形链球菌血清型f多糖激活人单核细胞:免疫球蛋白G Fc受体表达和肿瘤坏死因子及白介素-1的产生。

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Streptococcus mutans serotype f polysaccharide (poly f) was prepared from S. mutans whole cells by autoclaving. The poly f was purified by chromatography on DEAE Trisacryl M and Bio-Gel P100, treated with insoluble pronase, and resubjected to chromatography on DEAE Trisacryl M. Normal human blood monocytes, stimulated in vitro with purified poly f, produced extracellular tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in a dose-dependent fashion as determined by a heterologous two-site sandwich enzyme-linked immunosorbent assay. Poly f also increased the expression of monocyte cell surface receptors for the Fc part of human immunoglobulin G, activity which is correlated with an increase of the phagocytic activity of the stimulated monocytes. Polymyxin B had no effect on TNF-alpha and IL-1 beta release. Neutralization assays with anti-recombinant human TNF-alpha and anti-recombinant human IL-1 beta immunoglobulin G confirmed the fact that the cytotoxic and mitogenic mediators released by the poly f-stimulated monocytes were mainly TNF-alpha and IL-1 beta.
机译:通过高压灭菌自变形链球菌全细胞制备变形链球菌血清型f多糖(poly f)。通过在DEAE Trisacryl M和Bio-Gel P100色谱上纯化poly f,用不溶链酶处理,然后再在DEAE Trisacryl M上进行色谱分离。正常人血液单核细胞,在体外用纯化的poly f刺激,产生细胞外肿瘤坏死因子α TNF-α和白介素1β(IL-1 beta)呈剂量依赖性,这是通过异源两点夹心酶联免疫吸附试验确定的。聚f也增加了人免疫球蛋白G的Fc部分的单核细胞表面受体的表达,该活性与受刺激的单核细胞的吞噬活性的增加有关。多粘菌素B对TNF-α和IL-1β的释放没有影响。用抗重组人TNF-α和抗重组人IL-1β免疫球蛋白G进行的中和测定证实了由多f刺激的单核细胞释放的细胞毒性和促有丝分裂介质主要是TNF-α和IL-1β的事实。

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