...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli.
【24h】

Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli.

机译：质粒和染色体元件参与大肠杆菌附着和脱落的发病机理。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Attaching and effacing (A/E) intestinal lesions are produced by enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and RDEC-1, a pathogen of weanling rabbits. We recently identified a chromosomal locus (eae[E. coli A/E]) which is required for A/E activity in a wild-type EPEC strain. Sequences homologous to those of an eae gene probe were detected in EPEC, RDEC-1, and EHEC isolates. We report here that the eae gene is chromosomally encoded in all EPEC and EHEC strains tested and in RDEC-1. In addition, the eae probe was found to be 100% sensitive and 98% specific in detecting E. coli of EPEC serogroups that demonstrate A/E activity. Ten percent of E. coli of EPEC serogroups that hybridized with the eae probe and produced A/E activity did not hybridize with the EAF (EPEC adherence factor) probe, a plasmid-associated diagnostic probe which is currently used to identify EPEC. In addition to A/E factors, plasmid-associated adhesins also contribute to the pathogenesis of EPEC and RDEC-1. To further investigate the role of plasmid-associated adherence, a hybrid RDEC-1-EPEC strain containing the adherence plasmid of an EPEC strain in the A/E background of RDEC-1 was constructed. This hybrid strain, unlike the parent RDEC-1 strain, produced A/E lesions on human tissue culture cells, which suggests that the EPEC adherence plasmid provides tissue specificity to the hybrid strain and that the A/E factors of RDEC-1 are not host restricted.
机译：肠病原性大肠杆菌（EPEC），肠出血性大肠杆菌（EHEC）和断奶兔子的病原体RDEC-1产生附着和脱落的（A / E）肠道损伤。我们最近鉴定了野生型EPEC菌株中A / E活性所需的染色体基因座（eae [E。coli A / E]）。在EPEC，RDEC-1和EHEC分离物中检测到与eae基因探针同源的序列。我们在此报告，在所有测试的EPEC和EHEC菌株以及RDEC-1中，eae基因均是染色体编码的。此外，发现eae探针在检测表现出A / E活性的EPEC血清群的大肠杆菌中具有100％的敏感性和98％的特异性。与eae探针杂交并产生A / E活性的EPEC血清群的10％的大肠杆菌未与EAF（EPEC粘附因子）探针杂交，EAF是一种质粒相关的诊断探针，目前用于鉴定EPEC。除A / E因子外，质粒相关黏附素也有助于EPEC和RDEC-1的发病。为了进一步研究质粒相关粘附的作用，构建了在RDEC-1的A / E背景中包含EPEC菌株粘附质粒的杂种RDEC-1-EPEC菌株。与亲本RDEC-1菌株不同，该杂种菌株在人组织培养细胞上产生了A / E损伤，这表明EPEC粘附质粒为杂种菌株提供了组织特异性，而RDEC-1的A / E因子却没有主机受限。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号