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首页> 外文期刊>Infection and immunity >Urease-associated heat shock protein of Helicobacter pylori.
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Urease-associated heat shock protein of Helicobacter pylori.

机译：幽门螺杆菌的脲酶相关热休克蛋白。

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Helicobacter pylori urease is an extracellular, cell-bound enzyme with a molecular weight of approximately 600,000 (600K enzyme) comprising six 66K and six 31K subunits. A 62K protein is closely associated with the H. pylori urease, both in crude preparations and after gel filtration; this protein can be removed from the urease by ion-exchange chromatography without inactivating the enzyme. We purified this urease-associated protein and determined its N-terminal amino acid sequence. The sequence is 80% homologous (identical plus conserved amino acid residues) to the Escherichia coli GroEL heat shock protein (HSP), 75% homologous to the human homolog, and 84% homologous to the HSP homolog found in species of Chlamydia. Thus, the 62K urease-associated protein of H. pylori belongs to the HSP60 family of stress proteins known as chaperonins. Evidently this protein, HSP62, participates in the extracellular assembly and/or protection of the urease against inactivation in the hostile environment of the stomach.
机译：幽门螺杆菌脲酶是一种细胞外，细胞结合的酶，分子量约为600,000（600K酶），包含六个66K和六个31K亚基。无论是在粗制品中还是在凝胶过滤后，一种62K蛋白都与幽门螺杆菌脲酶密切相关。该蛋白可以通过离子交换色谱法从脲酶中去除，而不会使酶失活。我们纯化了这种脲酶相关蛋白，并确定了其N端氨基酸序列。该序列与大肠杆菌GroEL热休克蛋白（HSP）有80％的同源性（相同的氨基酸和保守的氨基酸残基），与人的同源物有75％的同源性，与衣原体中发现的HSP同源性有84％的同源性。因此，幽门螺杆菌的62K脲酶相关蛋白属于应激蛋白的HSP60家族，称为伴侣蛋白。显然，这种蛋白质HSP62参与了细胞外组装和/或尿素酶的保护，以防止在敌对的胃中失活。

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