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首页> 外文期刊>Infection and immunity >Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species.
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Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species.

机译：分析三种隐孢子虫卵囊壁和子孢子抗原。

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摘要

A comparison was made of the antigenic composition of oocyst walls and sporozoites from Cryptosporidium baileyi from turkeys, C. muris from rodents, and C. parvum from ruminants, employing immunoblotting and immunofluorescence. In immunoblotting, oocyst antigens were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) and detected with rabbit polyclonal anti-C. muris or -C. parvum antibodies or murine monoclonal antibodies developed against C. parvum. Immunofluorescence was used to investigate the reactivity of these monoclonal antibodies with air-dried excystation mixtures of sporozoites and oocysts of the different species. The results from both types of experiment indicated that the three Cryptosporidium species could be differentiated immunologically. In comparison, few antigenic differences were found between a number of isolates of C. parvum in immunoblotting. There was also evidence to suggest that C. parvum and C. baileyi were more closely related antigenically to one another than to C. muris.
机译：利用免疫印迹和免疫荧光比较了来自火鸡的百日隐孢子虫的卵囊壁和子孢子的抗原组成，来自啮齿动物的鼠毛衣原体和小反刍动物的抗原组成。在免疫印迹中，对卵囊抗原进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹（免疫印迹），并用兔多克隆抗C蛋白进行检测。 muris或-C。 parvum抗体或针对C. parvum的鼠单克隆抗体。免疫荧光用于研究这些单克隆抗体与不同物种的子孢子和卵囊的风干激发混合物的反应性。两种实验的结果表明，三种隐孢子虫都可以通过免疫学方法区分。相比之下，在免疫印迹中，许多小隐孢子虫分离株之间几乎没有抗原差异。也有证据表明，小球藻和百乐杆菌在抗原上的相互关系比与粘膜梭菌的关系更紧密。

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