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首页> 外文期刊>Infection and immunity >Serum antibody and cellular responses in LEW and F344 rats after immunization with Mycoplasma pulmonis antigens.
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Serum antibody and cellular responses in LEW and F344 rats after immunization with Mycoplasma pulmonis antigens.

机译：用肺炎支原体抗原免疫后LEW和F344大鼠的血清抗体和细胞应答。

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Mycoplasma pulmonis causes a chronic respiratory disease in rats which is more severe in LEW than in F344 rats. This study compared the ability of each of these rat strains to produce specific immune responses to M. pulmonis antigens. By an enzyme-linked immunosorbent assay, LEW rats were found to produce approximately 10 times lower levels of specific immunoglobulin G (IgG) after immunization with M. pulmonis antigens than F344 rats, while no significant difference was found in the levels of IgM. The difference in IgG levels was due to much greater levels of specific IgG2b (about 50 times) in F344 rats; no differences were found in other subclasses. Nonimmune LEW rats were found to have as much total IgG2b in their sera as unimmunized F344 rats by a single radial immunodiffusion test; thus, the difference was not due to the inability of LEW rats to produce IgG2b. In contrast to the antibody response to M. pulmonis antigens, anti-keyhole limpet hemocyanin IgG responses in LEW and F344 rats were similar, but F344 rats produced significantly more (about 21 times) IgG2b than was found in M. pulmonis responses. Antisera from F344 rats recognized several additional M. pulmonis antigens than antisera from LEW rats; however, this could not explain the differences in the level of IgG2b in LEW and F344 rats. In vitro stimulation of splenic lymphocytes with M. pulmonis antigens from immunized F344 rats produced much greater proliferative responses than in LEW and nonimmune F344 cells. Thus, the susceptible rat strain LEW produced lower cellular and humoral immune responses to M. pulmonis antigens than the resistant rat strain F344 after immunization.
机译：肺支原体引起大鼠的慢性呼吸系统疾病，在LEW中比在F344大鼠中更为严重。这项研究比较了每种大鼠品系对肺炎支原体抗原产生特异性免疫反应的能力。通过酶联免疫吸附试验，发现肺部鼠肺炎支原体抗原免疫后，LEW大鼠产生的特异性免疫球蛋白G（IgG）水平比F344大鼠低约10倍，而IgM水平则无明显差异。 IgG水平的差异是由于F344大鼠的特异性IgG2b水平高得多（约50倍）。在其他子类中未发现差异。通过单次径向免疫扩散试验，发现非免疫性LEW大鼠的血清中总IgG2b与未免疫F344大鼠相同。因此，差异不是由于LEW大鼠不能产生IgG2b。与针对肺炎支原体抗原的抗体反应相反，LEW和F344大鼠的抗匙孔血蓝蛋白IgG反应相似，但F344大鼠产生的IgG2b明显多于肺炎支原体反应（约21倍）。与来自LEW大鼠的抗血清相比，来自F344大鼠的抗血清还识别了几种肺炎支原体抗原。然而，这不能解释LEW和F344大鼠中IgG2b水平的差异。来自免疫的F344大鼠的肺炎支原体抗原体外刺激脾淋巴细胞产生的增殖反应比LEW和非免疫F344细胞产生的增殖反应要大得多。因此，免疫后，易感大鼠品系LEW对肺炎支原体抗原产生的细胞和体液免疫反应比抗性大鼠品系F344低。

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