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首页> 外文期刊>Infection and immunity >H-2 restriction in acquired cell-mediated immunity to infection with Francisella tularensis LVS.
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H-2 restriction in acquired cell-mediated immunity to infection with Francisella tularensis LVS.

机译：H-2限制获得性细胞介导的土拉弗朗西斯菌LVS感染的免疫力。

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The H-2 restriction imposed on the T-lymphocyte-macrophage interaction leading to the expression of acquired cellular immunity was evaluated in an experimental model of infection with the live vaccine strain of Francisella tularensis. Restriction between T cells and macrophages was examined in vitro in cultures containing macrophages from C57BL/10 (B10) mice, T cells from immune B10 H-2 congenic mice, and F. tularensis antigen. The cellular interaction was assayed by the production in the cultures of factors which stimulate thymocyte DNA synthesis. It was observed that homology at the I-A region of the H-2 complex was required for productive T-cell-macrophage cooperation to occur. Restriction was also investigated in an in vivo passive cell transfer system. Spleen cells from immunized B10 mice were injected into naive B10 H-2 congenic mice, which were then challenged with F. tularensis. Enhanced resistance to the challenge infection in recipient mice was used as a marker of a successful T-cell-macrophage interaction. It was found that when the recipient strain shared H-2 I-A region homology with the donor strain, enhanced antitularemic resistance was expressed, whereas homology at the H-2 K or D region was insufficient. Thus, macrophage--T-cell cooperation in immunity to experimental tularemia was restricted at the level of class II determinants.
机译：在图拉弗朗西斯菌活疫苗株感染的实验模型中评估了对T淋巴细胞-巨噬细胞相互作用导致获得的细胞免疫表达的H-2限制。在含有C57BL / 10（B10）小鼠巨噬细胞，来自免疫B10 H-2同基因小鼠的T细胞和T. tularensis抗原的培养物中，体外检测了T细胞和巨噬细胞之间的限制。通过刺激胸腺细胞DNA合成的因子的产生来测定细胞相互作用。观察到，H-2复合物的I-A区具有同源性是产生生产性T细胞-巨噬细胞合作所必需的。还在体内被动细胞转移系统中研究了限制。将来自免疫的B10小鼠的脾细胞注射到幼稚的B10 H-2同基因小鼠中，然后用土拉弗朗西斯菌攻击。受体小鼠对攻击性感染的增强抵抗力被用作成功的T细胞-巨噬细胞相互作用的标志。发现当受体菌株与供体菌株具有H-2 I-A区域同源性时，表达了增强的抗原核耐药性，而在H-2 K或D区域的同源性不足。因此，巨噬细胞-T细胞在针对实验性tularemia的免疫中的合作被限制在II类决定因素的水平。

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