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首页> 外文期刊>Infection and immunity >Major histocompatibility complex control of immunity elicited by genetically engineered Eimeria tenella (Apicomplexa) antigen in chickens.
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Major histocompatibility complex control of immunity elicited by genetically engineered Eimeria tenella (Apicomplexa) antigen in chickens.

机译：遗传改造的鸡艾美尔球虫（Apicomplexa）抗原引起的鸡免疫力的主要组织相容性复合物控制。

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The immunogenicity of a recombinant Eimeria tenella coccidial antigen was studied in 6(1).B congenic chickens derived from B2B2 and B5B5 parents segregating for haplotypes B2 and B5. Five-week-old chickens were immunized with 2.4 micrograms of recombinant protein (designated 5401) in Freund complete adjuvant and challenged with 75,000 oocysts at 28 days postimmunization (DPI) to determine the degree of elicited protective immunity. Serum samples were collected weekly for 5 weeks postimmunization for analysis by enzyme-linked immunosorbent assay, immunofluorescence assay, and Western blotting. Lesion scores following oocyst challenge were significantly reduced in B5B5 chickens compared with those in B2B2 chickens. Immunization induced a sporozoite-specific immunoglobulin G (IgG) titer in serum detected by the enzyme-linked immunosorbent assay that peaked at 28 DPI, the day of challenge, in B5B5 chickens and at 42 DPI in B2B2 chickens. After challenge, this titer declined for each genotype. Anti-sporozoite IgG detected by the immunofluorescence assay attained a peak titer at 21 DPI in B2B2 chickens and 28 DPI in B5B5 chickens. Serum from immunized B5B5 chickens reacted strongly in Western blots with several high-molecular-weight (greater than 100,000), soluble proteins prepared from sporozoites. Serum from B2B2 chickens reacted with similar proteins as well as with a 51- to 53-kilodalton protein that was not labeled by serum from B5B5 chickens. These results demonstrate further the role of host genetics on anticoccidial immunity and suggest that a peak anti-sporozoite IgG titer in B5B5 chickens on the day of challenge may signal a state of immunocompetence to that challenge.
机译：在6（1）.B中，从B2B2和B5B5亲本衍生的B型同源鸡分离出了单倍型B2和B5，研究了重组艾美尔球虫球虫抗原的免疫原性。用弗氏完全佐剂中的2.4微克重组蛋白（称为5401）对五周大的鸡进行免疫，并在免疫后28天（DPI）用75,000卵囊攻击，以确定引起的保护性免疫的程度。免疫后5周每周收集一次血清样品，以通过酶联免疫吸附测定，免疫荧光测定和蛋白质印迹进行分析。与B2B2鸡相比，B5B5鸡的卵囊攻击后病变评分显着降低。免疫诱导了血清中的子孢子特异性免疫球蛋白G（IgG）滴度，该滴度通过酶联免疫吸附试验检测到，在攻击当天，在B5B5鸡中达到28 DPI，在B2B2鸡中达到42 DPI。攻击后，每种基因型的滴度下降。通过免疫荧光分析检测到的抗子孢子IgG在B2B2鸡中达到21 DPI，在B5B5鸡中达到28 DPI的峰值滴度。来自免疫B5B5鸡的血清在Western印迹中与几种由子孢子制备的高分子量（大于100,000）可溶蛋白发生强烈反应。 B2B2鸡的血清与相似的蛋白质以及未由B5B5鸡的血清标记的51至53千达尔顿蛋白质反应。这些结果进一步证明了宿主遗传学对抗球虫免疫的作用，并表明在攻击当天B5B5鸡的抗子孢子IgG峰值达到峰值可能表明该攻击具有免疫能力。

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