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首页> 外文期刊>Infection and immunity >Inactivation of botulinum and tetanus toxins by chelators.
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Inactivation of botulinum and tetanus toxins by chelators.

机译：螯合剂灭活肉毒杆菌和破伤风毒素。

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Purified type A botulinum toxin of about 10(6) mouse 50% lethal doses per ml was greater than 99.9% inactivated when incubated at pH 7.4 for 30 min at 37 degrees C in 20 mM 1,10-phenanthroline (PTL) or 2,2'-dipyridyl (DPD) and was 96% inactivated when incubated in 70 mM 8-hydroxyquinoline-5-sulfonic acid (HQL), but was not affected when incubated in 200 mM EDTA. When used as a representative of the chelating agents, PTL inactivated greater than or equal to 99.9% of toxicity in the culture filtrate of C. botulinum type A, B, and E strains. Highly purified tetanus toxin at 2.5 x 10(5) 50% lethal doses per ml lost all toxicity in 40 mM PTL or 150 mM DPD but was not detectably affected by 100 mM HQL (the highest concentration possible). Toxin inactivation by 20 mM PTL was completely blocked when the PTL was prereacted with an equimolar amount of Zn2+ and significantly reduced when it was preincubated with one-third its molar amount of Fe2+. DPD at 20 mM had little toxin-inactivating potency when preincubated with an equimolar amount of Zn2+ and only some of this potency when preincubated with an equimolar amount of Fe2+. Toxicity was not recovered by adding Zn2+ or Fe2+ to PTL-treated toxin. Neutron activation analysis of type A toxin showed that for each toxin molecule present, there was 1 atom of Fe, 0.4 atom of Zn, and 22 to 55 atoms each of Ca and Mg. The biological activity of botulinum toxin seems to depend on a metal component, which is likely to be Fe.
机译：当在20毫升1,10-菲咯啉（PTL）或2中的pH 7.4下于37摄氏度下孵育30分钟时，每毫升约有10（6）只小鼠50％致死剂量的纯化的A型肉毒杆菌毒素被灭活大于99.9％。 2'-联吡啶（DPD）在70 mM 8-羟基喹啉-5-磺酸（HQL）中孵育时96％失活，但在200 mM EDTA中孵育时不受影响。当用作螯合剂的代表时，PTL灭活了A，B和E型肉毒梭菌菌株的培养滤液中大于或等于99.9％的毒性。每毫升2.5 x 10（5）50％致死剂量的高纯度破伤风毒素在40 mM PTL或150 mM DPD中失去了所有毒性，但不受100 mM HQL（可能的最高浓度）的影响。当PTL与等摩尔量的Zn2 +预反应时，通过20 mM PTL进行的毒素灭活被完全阻断，而当Pmol与摩尔量的Fe2 +的三分之一进行预温育时，毒素的失活则显着减少。当与等摩尔量的Zn2 +预孵育时，DPD在20 mM时几乎没有毒素灭活的能力，而与等摩尔量的Fe2 +预孵育时，只有部分这种毒素灭活的能力。通过向PTL处理的毒素中添加Zn2 +或Fe2 +不能恢复毒性。 A型毒素的中子活化分析表明，对于每个存在的毒素分子，Fe原子为1个，Zn为0.4个原子，Ca和Mg为22至55个原子。肉毒杆菌毒素的生物活性似乎取决于金属成分，该金属成分很可能是铁。

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