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首页> 外文期刊>Infection and immunity >Augmentation of the neutrophil response to Naegleria fowleri by tumor necrosis factor alpha.
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Augmentation of the neutrophil response to Naegleria fowleri by tumor necrosis factor alpha.

机译：肿瘤坏死因子α增强了中性粒细胞对家禽的致死反应。

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摘要

Conditioned medium from phytohemagglutinin-stimulated human mononuclear leukocytes, previously shown to activate neutrophils for amoeba killing, was found to contain high levels of tumor necrosis factor alpha (TNF-alpha) by an enzyme-linked immunosorbent assay. The effects of human recombinant TNF-alpha on the response of human neutrophils to the pathogenic free-living amoeba Naegleria fowleri was studied in vitro. The data showed that recombinant human TNF-alpha augmented the neutrophil respiratory burst (assessed by the cytochrome c reduction assay and lucigenin-dependent chemiluminescence assay) in response to amoebae opsonized with human serum. The priming effects of TNF-alpha were transient; marked enhancement was found with short 5- to 30-min preincubations of neutrophils with the cytokine. The enhancement of oxygen radical production was evident with 20 U of TNF-alpha per 10(6) neutrophils and continued to increase with up to 100 U. TNF-alpha also augmented the neutrophil lysosomal enzyme release in response to N. fowleri. The results support previous reports suggesting an important role of neutrophil cytokine activation for effective immunity against free-living amoebae.
机译：通过酶联免疫吸附试验发现，植物血凝素刺激的人单核白细胞的条件培养基先前显示可激活嗜中性粒细胞以杀灭变形虫，其条件培养基含有高水平的肿瘤坏死因子α（TNF-α）。体外研究了人类重组TNF-α对人类嗜中性粒细胞对病原性自由生存的变形虫Naegleria fowleri的反应的影响。数据显示，重组人TNF-α响应由人血清调理的变形虫，增强了嗜中性粒细胞呼吸爆发（通过细胞色素c还原测定和光泽精依赖性化学发光测定评估）。 TNF-α的启动作用是暂时的;嗜中性粒细胞与细胞因子预孵育5至30分钟后，发现明显增强。每10（6）个中性粒细胞20 U的TNF-α会明显增加氧自由基的产生，并随100 U的增加而持续增加。TNF-α还增加了对N. fowleri的中性粒细胞溶酶体酶的释放。该结果支持以前的报道，提示中性粒细胞细胞因子的激活对于有效抵抗自由活动的变形虫具有重要作用。

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