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首页> 外文期刊>Infection and immunity >Specificity of glycosphingolipid recognition by Entamoeba histolytica trophozoites.
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Specificity of glycosphingolipid recognition by Entamoeba histolytica trophozoites.

机译：溶脂囊虫滋养体糖鞘脂识别的特异性。

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The ability of purified glycosphingolipids to enhance liposome-stimulated Entamoeba histolytica actin polymerization was assessed as a means of defining the specificity of mammalian cell membrane lipid glycan recognition by this parasite. Synthetic liposomes containing a variety of individual glycosphingolipids bearing neutral, straight-chain oligomeric glycans with galactose or N-acetylgalactosamine termini stimulated rapid (90-s) polymerization of amoeba actin. Glycans with terminal N-acetylglucosamine residues were not stimulatory at all or were only weakly stimulatory. Glycans with glucose, N-acetylglucosamine, galactose, and N-acetylgalactosamine as the penultimate residue were recognized. Attachment of N-acetylneuraminate to the terminal residue of a stimulatory glycosphingolipid eliminated activity; attachment of fucose to the penultimate sugar reduced activity. Glycans with a terminal beta 1-4 or 1-3 glycosidic bond were most effective; glycans with terminal alpha 1-4 or 1-3 glycosides were less effective. The activity of glycans with both beta- and alpha-linked terminal glycosides was inhibited by lactose, suggesting recognition of both configurations by a single amoeba protein. The ability of liposomes to stimulate actin polymerization reflected the extent of liposome phagocytosis.
机译：评估纯化的鞘糖脂增强脂质体刺激的解脂变形杆菌（Entamoeba histolytica）肌动蛋白聚合反应的能力，作为定义该寄生虫对哺乳动物细胞膜脂质聚糖识别的特异性的手段。包含各种带有中性，直链寡聚聚糖和半乳糖或N-乙酰半乳糖胺末端的单个糖鞘脂的合成脂质体可刺激变形虫肌动蛋白快速（90-s）聚合。具有末端N-乙酰氨基葡糖残基的聚糖完全不刺激或仅弱刺激。识别了具有葡萄糖，N-乙酰氨基葡萄糖，半乳糖和N-乙酰半乳糖胺作为倒数第二个残基的聚糖。 N-乙酰神经氨酸在刺激性糖鞘脂消除活性的末端残基上的附着;将岩藻糖附着到倒数第二个糖上会降低活性。具有末端β1-4或1-3糖苷键的聚糖最有效。具有末端α1-4或1-3糖苷的聚糖效果较差。乳糖抑制具有β-和α-连接的末端糖苷的聚糖的活性，这表明单个变形虫蛋白可以识别两种构型。脂质体刺激肌动蛋白聚合的能力反映了脂质体吞噬作用的程度。

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