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首页> 外文期刊>Infection and immunity >New adhesive factor (antigen 8786) on a human enterotoxigenic Escherichia coli O117:H4 strain isolated in Africa.
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New adhesive factor (antigen 8786) on a human enterotoxigenic Escherichia coli O117:H4 strain isolated in Africa.

机译：在非洲分离的人产肠毒素的大肠杆菌O117：H4菌株上的新粘附因子（抗原8786）。

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摘要

An enterotoxigenic Escherichia coli strain, E. coli 8786, of serotype O117:H4 produced only heat-stable enterotoxin and gave mannose-resistant hemagglutination with human and bovine erythrocytes. The strain adhered to the brush border of human enterocytes and to enterocytelike cell line Caco-2. Adhesion inhibition assays using Caco-2 cells with different adhesive factor extracts showed that the adhesive factor of E. coli 8786 is different from colonization factor antigen I (CFA/I). CFA/II, CFA/III of Darfeuille et al. (A. Darfeuille, B. Lafeuille, B. Joly, and R. Cluzel, Ann. Microbiol. Inst. Pasteur 134A:53-64, 1983), CS6, and antigen 2230. A bacterial surface protein, designated antigen 8786, with a molecular mass of 16,300 Da was responsible for the adhesion to intestinal cells. It was immunologically different from previously described adhesive factors as determined by immunoblotting. Antigen 8786 was detected on the bacterial cell surface and appeared to be nonfimbrial. NH2-terminal analysis of antigen 8786 showed no homology with the previously described adhesive factors. Nevertheless, antigen 8786 is closely related to the NH2-terminal sequence of Salmonella enteritidis fimbrin. A hybridization experiment using a synthetic oligonucleotide probe based on the NH2-terminal amino acid sequence of antigen 8786 revealed that the coding region was located on a 70-MDa plasmid.
机译：O117：H4血清型的产肠毒素大肠杆菌菌株E. coli 8786仅产生热稳定的肠毒素，并产生人和牛红细胞对甘露糖的抗血凝作用。该菌株粘附于人肠上皮细胞的刷状缘和肠上皮样细胞系Caco-2。使用具有不同粘附因子提取物的Caco-2细胞进行粘附抑制试验表明，大肠杆菌8786的粘附因子不同于定居因子抗原I（CFA / I）。 Darfeuille等人的CFA / II，CFA / III。 （A.Darfeuille，B.Lafeuille，B.Joly和R.Cluzel，Ann.Microbiol.Inst.Pasteur 134A：53-64，1983），CS6和抗原2230。一种细菌表面蛋白，称为抗原8786，具有16300 Da的分子质量与肠细胞的粘附有关。通过免疫印迹测定，它在免疫学上不同于先前描述的粘附因子。在细菌细胞表面检测到抗原8786，似乎是非纤维的。抗原8786的NH2末端分析表明与先前描述的粘附因子没有同源性。不过，抗原8786与肠炎沙门氏菌纤维蛋白的NH2末端序列密切相关。使用基于抗原8786的NH2末端氨基酸序列的合成寡核苷酸探针进行的杂交实验表明，编码区位于70-MDa质粒上。

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