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首页> 外文期刊>Infection and immunity >Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent.
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Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent.

机译：对莱姆病病原体伯氏疏螺旋体的41-千屈鞭毛鞭毛抗原的体液反应的分子表征。

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摘要

The earliest humoral response in patients infected with Borrelia burgdorferi, the agent of Lyme disease, is directed against the spirochete's 41-kDa flagellar antigen. In order to map the epitopes recognized on this antigen, 11 overlapping fragments spanning the flagellin gene were cloned by polymerase chain reaction and inserted into an Escherichia coli expression vector which directed their expression as fusion proteins containing glutathione S-transferase at the N terminus and a flagellin fragment at the C terminus. Affinity-purified fusion proteins were assayed for reactivity on Western blots (immunoblots) with sera from patients with late-stage Lyme disease. The same immunodominant domain was bound by sera from 17 of 18 patients. This domain (comprising amino acids 197 to 241) does not share significant homology with other bacterial flagellins and therefore may be useful in serological testing for Lyme disease.
机译：感染莱姆病的伯氏疏螺旋体感染的患者，最早的体液反应是针对螺旋体的41 kDa鞭毛抗原。为了定位在该抗原上识别的表位，通过聚合酶链反应克隆了跨越鞭毛蛋白基因的11个重叠片段，并将其插入大肠杆菌表达载体，该载体指导它们表达为在N端含有谷胱甘肽S-转移酶的融合蛋白和C末端的鞭毛蛋白片段。亲和纯化的融合蛋白在Western blots（免疫印迹）上与晚期莱姆病患者的血清进行反应性测定。 18位患者中有17位的血清结合了相同的免疫优势结构域。该结构域（包含氨基酸197至241）与其他细菌鞭毛蛋白没有明显的同源性，因此可用于莱姆病的血清学检测。

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