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首页> 外文期刊>Infection and immunity >Structural and pathogenic properties of Aeromonas schubertii.
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Structural and pathogenic properties of Aeromonas schubertii.

机译：舒氏气单胞菌的结构和致病特性。

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We investigated the phenotypic, structural, and pathogenic properties of 11 Aeromonas schubertii strains recovered from extraintestinal sites. Most A. schubertii strains were autoagglutination positive, possessed a high surface charge but low hydrophobicity, and fell into one or two biogroups on the basis of carbon substrate utilization patterns. Fatty acid methyl ester analysis of A. schubertii revealed this species to contain a relatively high percentage of branched fatty acids (i-13:0, i-15:0, i-17:1, i-17:0) compared with A. hydrophila. Immunologic and biochemical analysis of the lipopolysaccharides of A. schubertii strains allowed for two groups to be distinguished, namely, (i) a collection of six strains belonging to serogroup O:11 that possessed a characteristic homogeneous O polysaccharide side chain profile by silver staining and immunoblotting techniques and (ii) a second antigenically diverse group (five strains) that either exhibited a heterogeneous side chain profile or were side chain deficient. A, schubertii O:11 strains were all found to contain a 55-kDa major protein associated with the outer membrane fraction which was glycine-hydrochloride extractable; non-O:11 strains did not harbor a similar protein molecule. Screening of A. schubertii strains for reputed virulence factors indicated (i) that slightly more than half of the isolates produce an apparent contact-dependent hemolysin that is not cell associated or released extracellularly, (ii) a potent cytotoxin active against HEp-2 cells that is devoid of hemolytic activity, and (iii) lack of enterotoxigeniclike activity as determined by suckling mouse assays. All A. schubertii strains were pathogenic for mice as determined by 50% lethal dose assays, although no single factor correlated with mouse pathogenicity.
机译：我们调查了从肠外部位恢复的11气单胞菌schubertii菌株的表型，结构和致病性。大部分舒氏曲霉菌株自凝集性阳性，具有高表面电荷但疏水性低，并且根据碳底物利​​用模式分为一个或两个生物组。与A相比，A. schubertii的脂肪酸甲酯分析显示该物种包含较高比例的支链脂肪酸（i-13：0，i-15：0，i-17：1，i-17：0）亲水性对schubertii菌株的脂多糖进行的免疫学和生化分析可以区分两个组，即（i）通过银染，收集了6个属于O：11血清型的菌株，这些菌株具有特征性的均一O多糖侧链谱。免疫印迹技术和（ii）第二个抗原多样性组（五个菌株），它们表现出异质的侧链谱或侧链缺陷。发现舒伯特氏菌O：11菌株均含有与外膜部分相关的55kDa主要蛋白质，该蛋白质可从甘氨酸盐酸盐中提取。 non-O：11菌株未包含类似的蛋白质分子。筛选A. schubertii菌株的致病力因子表明（i）稍多于一半的分离物会产生明显的接触依赖性溶血素，这种溶血素与细胞无关或不在细胞外释放;（ii）对HEp-2细胞有效的有效细胞毒素缺乏溶血活性，并且（iii）缺乏通过乳鼠试验确定的类肠毒素活性。尽管没有单一因素与小鼠致病性相关，但通过50％致死剂量测定法确定，所有schubertii菌株均对小鼠致病。

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