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Involvement of tumor necrosis factor alpha in intracellular multiplication of Legionella pneumophila in human monocytes.

机译：肿瘤坏死因子α参与人类单核细胞肺炎军团菌细胞内增殖。

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We investigated the role of tumor necrosis factor alpha (TNF-alpha) in human peripheral monocytes infected with Legionella pneumophila in vitro. Exogenous TNF-alpha significantly inhibited the intracellular multiplication of the bacterium. This effect was concentration and time dependent and was abrogated by anti-TNF antibodies. TNF-alpha levels in the culture supernatants were low but were enhanced by the addition of gamma interferon. When monocytes were cultured and infected in the presence of pentoxyphilline, a potent inhibitor of TNF-alpha synthesis, the intracellular bacterial growth was enhanced. The effect of pentoxyphilline was concentration and time dependent and was due to the inhibition of TNF-alpha production, as shown by Northern (RNA) blot hybridization of total RNA. In addition, the pentoxyphilline partially abolished the inhibitory effect of gamma interferon on bacterial intracellular multiplication. These results suggest that gamma interferon inhibits, at least partially, the intracellular multiplication of L. pneumophila by enhancing TNF-alpha synthesis.
机译：我们调查了肿瘤坏死因子α（TNF-alpha）在体外感染军团菌的人类外周血单核细胞中的作用。外源性TNF-α显着抑制细菌的细胞内繁殖。该作用是浓度和时间依赖性的，并被抗TNF抗体所消除。培养物上清液中的TNF-α水平较低，但可通过添加γ干扰素来提高。当培养单核细胞并在戊二酚（一种有效的TNF-α抑制剂）存在下感染时，细胞内细菌的生长得以增强。如总RNA的Northern（RNA）印迹杂交所显示，戊氧基苯胺的作用是浓度和时间依赖性的，并且归因于TNF-α的抑制。此外，戊氧亲啉部分消除了γ-干扰素对细菌细胞内增殖的抑制作用。这些结果表明，γ干扰素通过增强TNF-α合成来至少部分地抑制嗜肺乳杆菌的细胞内增殖。

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