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首页> 外文期刊>Infection and immunity >Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae.
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Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae.

机译：仔猪回肠粘液含有特异于大肠杆菌K88菌毛的蛋白质和糖脂（半乳糖神经酰胺）受体。

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The aim of this study was to characterize the Escherichia coli K88-specific receptors in mucus from the small intestines of 35-day-old piglets with the isogenic strains E. coli K-12(pMK005) (K88+) and E. coli K-12(pMK002) (K88-). These strains differed only in that the latter one cannot produce intact K88 fimbriae because of a deletion in the gene coding for the major fimbrial subunit. Adhesion was studied by incubating 3H-labeled bacteria with crude mucus, pronase-treated whole mucus, mucus fractionated by gel filtration, delipidated mucus, or extracted lipids immobilized in microtiter wells. In addition, E. coli strains were tested for adhesion to glycolipids extracted from mucus by overlaying glycolipid chromatograms with 125I-labeled bacteria. The recently reported finding that K88 fimbriae bind to glycoproteins in mucus from the piglet small intestine was confirmed in two ways. Pronase treatment of immobilized mucus reduced adhesion by 82%, and adhesion to delipidated mucus was 14 times greater for the K88+ than for the K88- strain. E. coli K88+ adhered to several of the fractions collected after gel filtration of crude mucus, including the void volume (M(r), > 250,000). Receptor activity specific for the K88 fimbriae was demonstrated in the lipids extracted from mucus, as the neutral lipids contained six times as much receptor activity as the acidic lipid fraction. Specificity was confirmed by demonstrating that adhesion to the total lipids could be inhibited by pretreatment of the immobilized lipids with K88 fimbriae. Relative to K-12 (K88-), the K-12 (K88+) bacterial cells bound more avidly to galactosylceramide when the neutral lipids were separated on thin-layer chromatography plates. No adhesion to lipids in the acidic fraction separated on thin-layer plates was detected. Relative to adhesion of K-12 (K88-), adhesion of K-12 (K88+) to commercially available galactosylceramide immobilized in microtiter wells confirmed the results with the thin-layer plates. It can be concluded that 35-day-old piglet mucus contains both protein and glycolipid receptors specific for K88 fimbriae, the latter being galactosylceramide.
机译：这项研究的目的是鉴定具有等基因菌株E. coli K-12（pMK005）（K88 +）和E. coli K-的35日龄仔猪小肠粘液中的大肠杆菌K88特异性受体12（pMK002）（K88-）。这些菌株的不同之处仅在于，由于编码主要纤维亚基的基因缺失，后一种菌株无法产生完整的K88菌毛。通过将3H标记的细菌与粗粘液，链霉蛋白酶处理过的完整粘液，通过凝胶过滤分级的粘液，脱脂的粘液或固定在微量滴定孔中的提取脂质一起孵育来研究粘附力。此外，通过将糖脂色谱图与125 I标记细菌重叠，测试了大肠杆菌菌株对从粘液中提取的糖脂的粘附性。最近报道的发现以两种方式证实了K88菌毛结合仔猪小肠粘液中的糖蛋白。链霉菌蛋白酶对固定化粘液的粘附力降低了82％，而对于K88 +菌株，对脱脂粘液的粘附力是K88-菌株的14倍。大肠杆菌K88 +粘附到粗粘液凝胶过滤后收集的部分馏分上，包括空隙体积（M（r），> 250,000）。在从粘液中提取的脂质中证明了对K88菌毛特异的受体活性，因为中性脂质所含的受体活性是酸性脂质组分的六倍。通过证明用K88菌毛预处理固定的脂质可以抑制对总脂质的粘附，从而证实了特异性。相对于K-12（K88-），当在薄层色谱板上分离中性脂质时，K-12（K88 +）细菌细胞与半乳糖神经酰胺的结合更加亲和。在薄板上分离的酸性级分中未检测到对脂质的粘附。相对于K-12（K88-）的粘附力，K-12（K88 +）与固定在微量滴定孔中的市售半乳糖神经酰胺的粘附力证实了薄板的结果。可以得出结论，35天大的仔猪粘液同时含有K88菌毛特异的蛋白质和糖脂受体，后者是半乳糖基神经酰胺。

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