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首页> 外文期刊>Infection and immunity >Alteration of the pilin adhesin of Pseudomonas aeruginosa PAO results in normal pilus biogenesis but a loss of adherence to human pneumocyte cells and decreased virulence in mice.
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Alteration of the pilin adhesin of Pseudomonas aeruginosa PAO results in normal pilus biogenesis but a loss of adherence to human pneumocyte cells and decreased virulence in mice.

机译：铜绿假单胞菌PAO的菌毛粘附素的改变导致正常的菌毛生物发生，但是丧失了对人肺细胞的粘附并降低了小鼠的毒力。

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摘要

The disulfide loop domain of Pseudomonas aeruginosa PAO pilin was altered by insertion of a chloramphenicol acetyltransferase gene into the pilin gene so that the C-terminal nine amino acids were replaced with 11 new amino acids. The altered pilin gene was transferred into wild-type PAO by recombination, where it did not affect normal piliation as observed by transmission electron microscopy or change of sensitivity to f116, PO4, B9, and Pf1 pilus-specific bacteriophages. However, the binding to human pneumocyte A549 cells was markedly reduced when tested in an in vitro binding assay (2 to 6 bacteria bound per A549 cell for the mutant bacteria compared with 50 bacteria per A549 cell for the wild-type bacteria). Additionally, when susceptible A.BY/SnJ mice were challenged with wild-type P. aeruginosa PAO and with P. aeruginosa PAO-MP (altered pilin gene), a 50% lethal dose of 3 x 10(6) bacteria per mouse was observed for PAO-MP compared with 7 x 10(4) bacteria per mouse for PAO. Approximately 90 of the adherence capability of P. aeruginosa PAO is seemingly attributable to the C-terminal disulfide loop adherence domain of pili. The pilus adherence function contributes significantly to the virulence of P. aeruginosa PAO in the A.BY/SnJ mouse infection model.
机译：铜绿假单胞菌PAO菌毛素的二硫环结构域通过将氯霉素乙酰基转移酶基因插入菌毛素基因中而改变，从而C端的9个氨基酸被11个新的氨基酸取代。改变的菌毛蛋白基因通过重组被转移到野生型PAO中，通过透射电子显微镜或对f116，PO4，B9和Pf1菌毛特异性噬菌体的敏感性变化，它不会影响正常的菌毛。但是，当在体外结合试验中进行测试时，与人肺细胞A549细胞的结合显着降低（突变菌每个A549细胞结合2至6个细菌，而野生型菌每个A549细胞则结合50个细菌）。此外，当易感性A.BY/SnJ小鼠用野生型铜绿假单胞菌PAO和铜绿假单胞菌PAO-MP（改变的菌毛蛋白基因）攻击时，每只小鼠的50％致死剂量的3 x 10（6）细菌是致命的。观察到的PAO-MP与每只小鼠的7 x 10（4）细菌比较。铜绿假单胞菌PAO的大约90的粘附能力似乎归因于菌毛的C端二硫键环粘附域。在A.BY/SnJ小鼠感染模型中，菌毛的粘附功能对铜绿假单胞菌PAO的毒力有显着贡献。

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