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首页> 外文期刊>Infection and immunity >Characterization of an antiproliferative surface-associated protein from Actinobacillus actinomycetemcomitans which can be neutralized by sera from a proportion of patients with localized juvenile periodontitis.
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Characterization of an antiproliferative surface-associated protein from Actinobacillus actinomycetemcomitans which can be neutralized by sera from a proportion of patients with localized juvenile periodontitis.

机译：放线放线杆菌的抗增殖表面相关蛋白的表征，可被一部分局部性青少年牙周炎患者的血清中和。

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The gentle agitation of suspensions of Actinobacillus actinomycetemcomitans serotype a, b, or c in saline resulted in the release of a proteinaceous surface-associated material (SAM) which produced a dose-dependent inhibition of tritiated thymidine incorporation by the osteoblast-like cell line MG63 in culture. This cell line was sensitive to low concentrations of SAM (50% inhibitory concentration, 200 ng/ml for serotype c). Immunoglobulin G antibodies to constituents of the SAM were found in the blood of patients with localized juvenile periodontitis (LJP). Sera from 9 of 16 patients with LJP significantly neutralized the antiproliferative activity of the SAM, while sera from 15 controls, with no evidence of periodontal disease, were unable to neutralize this activity. Neutralization was not directly related to the patient's antibody titer to the whole SAM. Characterization of the antiproliferative activity in the SAM demonstrated that it was not cytotoxic and was heat and trypsin sensitive. The active component separated in a well-defined peak in anion-exchange high-performance liquid chromatography (HPLC) which, when further analyzed by size exclusion HPLC, revealed a single active peak, which had an apparent molecular mass of approximately 8 kDa. The lipopolysaccharide from A. actinomycetemcomitans was only weakly active. SAM from Porphyromonas gingivalis W50 and Eikenella corrodens NCTC 10596 did not exhibit any antiproliferative activity with this cell line, even at concentrations as high as 10 micrograms/ml. This study has shown that SAM from A. actinomycetemcomitans contains a potent antiproliferative protein whose activity can be neutralized by antibodies in the sera from some patients with LJP.
机译：在盐水中轻度搅拌放线放线杆菌放线杆菌a，b或c血清型的悬浮液，导致释放出蛋白质表面相关物质（SAM），从而对成骨细胞样细胞系MG63 tri化胸苷掺入产生剂量依赖性抑制作用在文化中。该细胞系对低浓度的SAM敏感（50％抑制浓度，血清型c为200 ng / ml）。在局部少年牙周炎（LJP）患者的血液中发现了针对SAM成分的免疫球蛋白G抗体。 16名LJP患者中有9名血清显着中和了SAM的抗增殖活性，而15名对照中没有牙周疾病的证据的血清无法中和该活性。中和与患者对整个SAM的抗体效价没有直接关系。 SAM中抗增殖活性的表征表明，它没有细胞毒性，对热和胰蛋白酶敏感。在阴离子交换高效液相色谱（HPLC）中，活性成分分离在一个明确定义的峰中，当通过尺寸排阻HPLC进一步分析时，该活性成分显示出一个单个的活性峰，其表观分子量约为8 kDa。来自放线放线杆菌的脂多糖仅是弱活性的。来自牙龈卟啉单胞菌W50和腐蚀的艾肯氏菌NCTC 10596的SAM即使在浓度高达10微克/毫升的情况下也没有表现出任何抗增殖活性。这项研究表明，来自放线放线杆菌的SAM包含一种有效的抗增殖蛋白，其活性可以被某些LJP患者血清中的抗体所中和。

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