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首页> 外文期刊>Infection and immunity >Rapid and complete fusion of macrophage lysosomes with phagosomes containing Salmonella typhimurium.
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Rapid and complete fusion of macrophage lysosomes with phagosomes containing Salmonella typhimurium.

机译：快速和完全融合巨噬细胞溶酶体与鼠伤寒沙门氏菌的吞噬体。

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The virulence of Salmonella typhimurium for mice results, in part, from its ability to survive after phagocytosis by macrophages. Although it is generally agreed that intracellular bacteria persist in membrane-bound phagosomes, there remains some question as to whether these phagosomes fuse with macrophage lysosomes. This report describes the maturation of phagosomes containing S. typhimurium inside mouse bone marrow-derived macrophages. Macrophages were infected briefly and incubated for various intervals; then they were examined by fluorescence microscopy for colocalization of bacteria with lysosomal markers. These markers included LAMP-1, cathepsin L, and fluorescent proteins or dextrans preloaded into lysosomes by endocytosis. By all measures, phagosomes containing S. typhimurium merged completely with the lysosomal compartment within 20 min of phagocytosis. The rate of phagosome-lysosome fusion was similar to the rate for phagocytosed latex beads. Phagolysosomes remained accessible to fluid-phase probes and contained lysosomal markers for many hours. Moreover, a large percentage of the wild-type bacteria that were viable 20 min after infection survived longer incubations inside macrophages, indicating that the survivors were not a minor subpopulation that avoided phagosome-lysosome fusion. Therefore, we conclude that S. typhimurium survives within the lysosomal compartments of macrophages.
机译：鼠伤寒沙门氏菌对小鼠的毒力部分是由巨噬细胞吞噬后的存活能力造成的。尽管通常认为细胞内细菌在膜结合的吞噬体中持续存在，但是关于这些吞噬体是否与巨噬细胞溶酶体融合仍然存在一些问题。该报告描述了小鼠骨髓来源的巨噬细胞中含有鼠伤寒沙门氏菌的吞噬体的成熟。短暂感染巨噬细胞并孵育不同的时间间隔;然后通过荧光显微镜检查它们与溶酶体标记物共定位的细菌。这些标记包括LAMP-1，组织蛋白酶L和通过胞吞作用预加载到溶酶体中的荧光蛋白或右旋糖酐。通过所有措施，在吞噬作用的20分钟内，含有鼠伤寒沙门氏菌的吞噬体与溶酶体区室完全融合。吞噬体-溶酶体融合的速率与吞噬乳胶珠的速率相似。吞噬酶体仍可被液相探针接触，并含有溶酶体标记许多小时。此外，感染后20分钟仍能存活的大部分野生型细菌在巨噬细胞内的孵育时间更长，这表明幸存者不是避免吞噬体-溶酶体融合的次要种群。因此，我们得出结论，鼠伤寒沙门氏菌在巨噬细胞的溶酶体区室中存活。

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