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首页> 外文期刊>Infection and immunity >Effects of Ixodes scapularis and Borrelia burgdorferi on modulation of the host immune response: induction of a TH2 cytokine response in Lyme disease-susceptible (C3H/HeJ) mice but not in disease-resistant (BALB/c) mice.
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Effects of Ixodes scapularis and Borrelia burgdorferi on modulation of the host immune response: induction of a TH2 cytokine response in Lyme disease-susceptible (C3H/HeJ) mice but not in disease-resistant (BALB/c) mice.

机译：肩x骨和伯氏疏螺旋体对宿主免疫应答的调节作用：在易感莱姆病（C3H / HeJ）的小鼠中诱导TH2细胞因子应答，而在抗病性（BALB / c）的小鼠中则没有。

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Previous studies have demonstrated that both Ixodes scapularis saliva and Borrelia burgdorferi antigens modulated lymphokines and monokines in vitro. The studies presented here were designed to delineate the role of I. scapularis and B. burgdorferi in modulation of the host immune response in vivo. Infestation of C3H/HeJ mice with infected I. scapularis resulted in an up regulation of IL-4 as early as 8 days after tick infestation, while the levels of T helper cell type 1 (TH1) cytokines, interleukin-2 (IL-2) and gamma interferon (IFN-gamma), were significantly decreased by days 10 to 12. In contrast, the cytokine profile of BALB/c mice exposed to infected nymphal ticks resulted in only transient alterations in IL-4, IL-2, and IFN-gamma production throughout a 12-day period postinfestation. Although the IL-10 level was elevated in both C3H/HeJ and BALB/c mice infested with infected nymphal ticks, no significant difference in the levels of IL-10 was noted between the mouse strains. Flow-cytometric analysis demonstrated increases in the numbers of splenic B-cell and CD4+ lymphocytes in C3H/HeJ but not BALB/c mice exposed to infected ticks. Cell depletion experiments with C3H/HeJ mice demonstrated that CD4+ cells were the sole producers of IFN-gamma and IL-10 while both CD4+ and CD8+ splenocytes contributed to the production of IL-2 and IL-4. These findings suggest that B and CD4+ splenocytes are activated, increase in number, and produce a polarized TH2 response in C3H/HeJ mice exposed to infected I. scapularis. Given that C3H/HeJ mice are susceptible to Lyme disease and the initial TH2 polarization is not evident in BALB/c mice, effective control of this response may have ramifications for spirochete transmission in vivo.
机译：先前的研究表明，肩x小唾液和伯氏疏螺旋体抗原均在体外调节淋巴因子和单核因子。本文介绍的研究旨在描述肩cap骨和伯氏疏螺旋体在体内调节宿主免疫应答中的作用。 C3H / HeJ小鼠感染肩炎后，在tick虫感染后8天之内就​​导致IL-4上调，而T辅助细胞1型（TH1）细胞因子白介素2（IL-2）的水平升高。 ）和γ干扰素（IFN-γ）在第10天到第12天显着降低。相反，暴露于感染的若虫tick的BALB / c小鼠的细胞因子谱仅导致IL-4，IL-2和感染后整整12天的IFN-γ产生。尽管在感染了若虫壁虱的C3H / HeJ和BALB / c小鼠中IL-10水平均升高，但是在小鼠品系之间未观察到IL-10水平的显着差异。流式细胞仪分析表明暴露于受感染s的C3H / HeJ小鼠脾脏B细胞和CD4 +淋巴细胞数量增加，而BALB / c小鼠则没有。用C3H / HeJ小鼠进行的细胞耗竭实验表明，CD4 +细胞是IFN-γ和IL-10的唯一产生者，而CD4 +和CD8 +脾细胞均有助于IL-2和IL-4的产生。这些发现表明，B和CD4 +脾细胞被激活，数量增加，并在暴露于受感染的肩骨的C3H / HeJ小鼠中产生极化的TH2反应。鉴于C3H / HeJ小鼠易感莱姆病，而在BALB / c小鼠中初始TH2极化不明显，因此对该反应的有效控制可能会对螺旋体在体内的传播产生影响。

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