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首页> 外文期刊>Infection and immunity >Expression of Virulence of Mycobacterium tuberculosiswithin Human Monocytes: Virulence Correlates with Intracellular Growth and Induction of Tumor Necrosis Factor Alpha but Not with Evasion of Lymphocyte-Dependent Monocyte Effector Functions
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Expression of Virulence of Mycobacterium tuberculosiswithin Human Monocytes: Virulence Correlates with Intracellular Growth and Induction of Tumor Necrosis Factor Alpha but Not with Evasion of Lymphocyte-Dependent Monocyte Effector Functions

机译：人单核细胞中结核分枝杆菌毒力的表达：毒力与细胞内生长和肿瘤坏死因子α的诱导相关，但与淋巴细胞依赖性单核细胞效应子功能的逃逸无关。

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We assessed the applicability of an in vitro model of low-level infection of human monocytes to the characterization of the virulence of strains of the Mycobacterium tuberculosis family. Peripheral blood monocytes were infected at a 1:1 ratio with the virulent M. tuberculosis strain H37Rv, the avirulentM. tuberculosis strain H37Ra, and the attenuated M. bovis strain BCG. Both the percentages of cells infected by the three strains and the initial numbers of intracellular organisms were equivalent, as were levels of monocyte viability up to 7 days following infection. Intracellular growth reflected virulence, as H37Rv replicated in logarithmic fashion throughout the assay, BCG growth reached a plateau at 4 days, and H37Ra did not grow at all. The same patterns of growth were observed following infection of human alveolar macrophages with H37Rv and H37Ra. Monocyte production of tumor necrosis factor alpha was significantly higher following infection with virulent H37Rv than with either BCG or H37Ra. In contrast, there was no clear correlation of interleukin 10 production with virulence. Nonadherent cells of purified-protein-derivative-positive donors mediated equivalent degrees of reduction of the intracellular growth of H37Rv, BCG, and H37Ra. Low-level infection of human monocytes with H37Rv, BCG, and H37Ra thus provides an in vitro model for assessment of the virulence of these M. tuberculosis family strains. Furthermore, it is suggested that the virulence of these strains is expressed primarily by their differing abilities to adapt to the intracellular environment of the mononuclear phagocyte.
机译：我们评估了人类单核细胞低水平感染的体外模型对结核分枝杆菌家族毒力的表征的适用性。外周血单核细胞以1：1的比例感染有毒的 M。结核菌株H37Rv，无毒的 M。肺结核菌株H37Ra和减毒的 M。牛卡介苗株BCG。三种菌株感染的细胞百分比和细胞内生物的初始数目都相等，感染后长达7天的单核细胞活力水平也相同。细胞内生长反映出毒力，因为在整个检测过程中H37Rv以对数方式复制，BCG生长在4天达到平稳，而H37Ra根本不生长。用H37Rv和H37Ra感染人肺泡巨噬细胞后，观察到相同的生长方式。毒性H37Rv感染后，肿瘤坏死因子α的单核细胞产生显着高于BCG或H37Ra。相反，白介素10的产生与毒力之间没有明确的相关性。纯化的蛋白质衍生物阳性供体的非贴壁细胞介导了H37Rv，BCG和H37Ra的细胞内生长降低的程度相同。因此，H37Rv，BCG和H37Ra对人单核细胞的低水平感染为评估这些 M的毒力提供了一种体外模型。结核家族菌株。此外，建议这些菌株的毒力主要由它们适应单核吞噬细胞的细胞内环境的不同能力表达。

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