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首页> 外文期刊>Infection and immunity >Antibody Response to Fibronectin-Binding Adhesin FnbpA in Patients with Staphylococcus aureusInfections
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Antibody Response to Fibronectin-Binding Adhesin FnbpA in Patients with Staphylococcus aureusInfections

机译：金黄色葡萄球菌感染患者对纤连蛋白结合黏附蛋白FnbpA的抗体反应

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摘要

We have analyzed antibody reactivity to a fibronectin-binding microbial surface component that recognizes adhesive matrix molecules (MSCRAMM) in blood plasma collected from patients with staphylococcal infections. All patients had elevated levels of anti-MSCRAMM antibodies compared to those of young children who, presumably, had not been exposed to staphylococcal infections. The anti-MSCRAMM antibodies preferentially reacted with the ligand-binding repeat domain of the adhesin. However, these antibodies did not inhibit fibronectin binding. Essentially, all patients had antibodies which specifically recognized the fibronectin-MSCRAMM complex but not the isolated components. Epitopes recognized by these anti-ligand-induced binding sites antibodies were found in each repeat unit of the MSCRAMM. These results demonstrate that staphylococci have bound fibronectin some time during infection and that each repeat unit in the MSCRAMM can engage in ligand binding. Furthermore, our previously proposed model, suggesting that an unordered structure in the MSCRAMM undergoes a conformational change upon ligand binding (K. House-Pompeo, Y. Xu, D. Joh, P. Speziale, and M. H??k, J. Biol. Chem. 271:1379–1384, 1996), is presumably operational in patients during infections.
机译：我们已经分析了与纤连蛋白结合微生物表面成分的抗体反应性，该成分可识别从葡萄球菌感染患者收集的血浆中的粘附基质分子（MSCRAMM）。与据推测未暴露于葡萄球菌感染的幼儿相比，所有患者的抗MSCRAMM抗体水平均升高。抗MSCRAMM抗体优先与粘附素的配体结合重复域反应。但是，这些抗体不抑制纤连蛋白结合。基本上，所有患者的抗体均能特异性识别纤连蛋白-MSCRAMM复合物，但不能识别分离出的成分。在MSCRAMM的每个重复单元中都发现了被这些抗配体诱导的结合位点抗体识别的表位。这些结果表明，葡萄球菌在感染过程中已经结合了纤连蛋白，并且MSCRAMM中的每个重复单元都可以参与配体结合。此外，我们先前提出的模型表明，MSCRAMM中的无序结构在配体结合后发生构象变化（K. House-Pompeo，Y. Xu，D. Joh，P. Speziale，and M. H ?? k，J （Biol。Chem。271：1379-1384，1996），大概是在感染期间对患者有效的。

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