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首页> 外文期刊>Infection and immunity >Shiga Toxin 1 from Escherichia coliBlocks Activation and Proliferation of Bovine Lymphocyte Subpopulations In Vitro
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Shiga Toxin 1 from Escherichia coliBlocks Activation and Proliferation of Bovine Lymphocyte Subpopulations In Vitro

机译：大肠杆菌的志贺毒素1阻止体外牛淋巴细胞亚群的激活和增殖

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Shiga toxin-producing Escherichia coli (STEC) is widespread in the cattle population, but the clinical significance of Shiga toxins (Stx’s) for the bovine species remains obscure. Since Stx’s exert immunomodulating effects in other species, we examined the effect of purified Stx1 on a bovine B lymphoma cell line (BL-3) and peripheral blood mononuclear cells (PBMC) isolated from adult bovine blood by viability assays and flow cytometry analysis. Stx1 markedly induced apoptosis in stimulated BL-3 cells. The susceptibility of this B-cell-derived cell line was induced only by either lipopolysaccharide (LPS) or pokeweed mitogen, while cultures stimulated with T-cell mitogens were unaffected by the toxin. In contrast, Stx1 did not induce cellular death—neither apoptosis nor necrosis—in primary cultures of PBMC but hindered the mitogen-induced increase in metabolic activity. The influence of Stx1 on single PBMC subpopulations varied with the type of mitogenic stimulus applied. Stimulation with phytohemagglutinin P particularly induced the proliferation of bovine CD8-expressing (BoCD8+) cells, and this proliferative response was blocked by Stx1. On the other hand, Stx1 reduced the portion of viable B cells in the presence of LPS. Modulation of activation marker expression (BoCD25 and BoCD71) by Stx1 indicated that the toxin hindered the proliferation of cells by blocking their activation. In conclusion, we assume that Stx1 contributes to the pathogenesis of STEC-associated diarrhea in calves by suppressing the mucosa-associated immune response. The usefulness of cattle as a model in which to study Stx-induced immunomodulation is discussed.
机译：产生志贺毒素的大肠埃希菌（STEC）在牛群中很普遍，但是对于牛物种来说，志贺毒素（Stx's）的临床意义仍然不清楚。由于Stx在其他物种中发挥免疫调节作用，因此我们通过生存力分析和流式细胞术分析了纯化的Stx1对从成年牛血中分离的牛B淋巴瘤细胞系（BL-3）和外周血单个核细胞（PBMC）的影响。 Stx1明显诱导刺激的BL-3细胞凋亡。这种B细胞来源的细胞系仅由脂多糖（LPS）或商陆有丝分裂原诱导，而由T细胞有丝分裂原刺激的培养物不受毒素影响。相比之下，Stx1在PBMC的原代培养中没有诱导细胞死亡（凋亡或坏死都没有），但阻碍了促分裂原诱导的代谢活性的增加。 Stx1对单个PBMC亚群的影响随所施加的有丝分裂刺激的类型而异。植物血凝素P的刺激特别诱导了表达牛CD8的（BoCD8 + ）细胞的增殖，Stx1阻止了这种增殖反应。另一方面，在存在LPS的情况下，Stx1减少了存活的B细胞的比例。 Stx1对激活标志物表达（BoCD25和BoCD71）的调节表明该毒素通过阻止其激活来阻止细胞的增殖。总之，我们认为Stx1通过抑制粘膜相关的免疫反应而促进了犊牛STEC相关的腹泻的发病机理。讨论了牛作为研究Stx诱导的免疫调节模型的有用性。

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