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Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

机译：胶囊阻碍肺炎克雷伯菌的粘附和侵袭上皮细胞

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The adhesion of K21a, K26, K36, and K50 capsulatedKlebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistantKlebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization ofKlebsiella.
机译：K21a，K26，K36和K50封装的 Klebsiella 菌株对回盲菌（HCT-8）和膀胱（T24）上皮细胞系的粘附力显着低于其相应的自发非封装变体K21a / 3 ，K26 / 1，K36 / 3和K50 / 3。两种上皮细胞系对细菌的内在化也显着减少。类似地，与K2亲本菌株相比，胶囊转换的衍生物K2（K36）表现出形态更大的K36胶囊，并且形成的荚膜物质侵入回盲肠上皮细胞系的能力较差。没有一个封装的菌株表现出显着的甘露糖敏感的1型菌毛，而两个非封装的变体K21a / 3和K50 / 3却表现出强大的甘露糖敏感的血凝活性。尽管归因于抗甘露糖的3型克雷伯氏菌3型菌毛的血凝活性在所有菌株中均较弱，但在某些情况下，封装的亲本菌株的滴度低于其相应的非封装变体。尽管对回盲细胞的粘附水平与对膀胱细胞的粘附程度没有区别，但膀胱细胞对细菌的内化作用明显低于对回肠细胞的内化作用，这表明膀胱细胞缺乏克雷伯菌内在化所必需的成分。

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