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首页> 外文期刊>Infection and immunity >Genetic Structure and Distribution of Four Pathogenicity Islands (PAI I536 to PAI IV536) of Uropathogenic Escherichia coli Strain 536
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Genetic Structure and Distribution of Four Pathogenicity Islands (PAI I536 to PAI IV536) of Uropathogenic Escherichia coli Strain 536

机译：致病性大肠杆菌536菌株的四个致病岛（PAI I536至PAI IV536）的遗传结构和分布

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For the uropathogenic Escherichia coli strain 536 (O6:K15:H31), the DNA sequences of three pathogenicity islands (PAIs) (PAI I536 to PAI III536) and their flanking regions (about 270 kb) were determined to further characterize the virulence potential of this strain. PAI I536 to PAI III536 exhibit features typical of PAIs, such as (i) association with tRNA-encoding genes; (ii) G+C content differing from that of the host genome; (iii) flanking repeat structures; (iv) a mosaic-like structure comprising a multitude of functional, truncated, and nonfunctional putative open reading frames (ORFs) with known or unknown functions; and (v) the presence of many fragments of mobile genetic elements. PAI I536 to PAI III536 range between 68 and 102 kb in size. Although these islands contain several ORFs and known virulence determinants described for PAIs of other extraintestinal pathogenic E. coli (ExPEC) isolates, they also consist of as-yet-unidentified ORFs encoding putative virulence factors. The genetic structure of PAI IV536, which represents the core element of the so-called high-pathogenicity island encoding a siderophore system initially identified in pathogenic yersiniae, was further characterized by sample sequencing. For the first time, multiple PAI sequences (PAI I536 to PAI IV536) in uropathogenic E. coli were studied and their presence in several wild-type E. coli isolates was extensively investigated. The results obtained suggest that these PAIs or at least large fragments thereof are detectable in other pathogenic E. coli isolates. These results support our view that the acquisition of large DNA regions, such as PAIs, by horizontal gene transfer is an important factor for the evolution of bacterial pathogens.
机译：对于泌尿致病性大肠埃希菌536（O6：K15：H31），其三个致病岛（PAI I 536 至PAI III 536）的DNA序列）及其侧翼区域（约270 kb），以进一步表征该菌株的潜在毒力。 PAI I 536 至PAI III 536 具有PAI的典型特征，例如（i）与编码tRNA的基因缔合; （ii）G + C含量不同于宿主基因组的含量; （iii）侧翼重复结构; （iv）马赛克状结构，包括多个具有已知或未知功能的功能性，截短性和非功能性推定开放阅读框（ORF）; （v）存在许多移动遗传元件的片段。 PAI I 536 到PAI III 536 的大小在68到102 kb之间。尽管这些岛包含多个ORF和其他肠道外致病性Eem的PAI所描述的已知毒力决定因素。大肠杆菌（ExPEC）分离株，它们还由编码推定毒力因子的尚未确定的ORF组成。通过样品测序进一步表征了PAI IV 536 的遗传结构，该结构代表所谓的高致病性岛的核心元素，该岛编码最初在致病性耶尔森氏菌中鉴定的铁载体系统。尿毒症致病性 E中首次出现多个PAI序列（PAI I 536 至PAI IV 536 ）。研究了大肠杆菌及其在几种野生型 E中的存在。对大肠杆菌分离株进行了广泛的研究。获得的结果表明，这些PAI或其至少大片段可在其他病原体E中检测到。大肠杆菌分离株。这些结果支持我们的观点，即通过水平基因转移获得大的DNA区域（例如PAI）是细菌病原体进化的重要因素。

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