...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus
【24h】

Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus

机译：生物膜相关蛋白Bap在牛金黄色葡萄球菌发病机理中的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Staphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability of the bacteria to produce biofilm. Here, we report a relationship between the ability to produce chronic bovine mastitis and biofilm formation. We have classified bovine mastitis S. aureus isolates into three groups based on the presence of particular genetic elements required for biofilm formation: group 1 (ica+ bap+), group 2 (ica+, bap negative), and group 3 (ica negative, bap negative). Overall, animals naturally infected with group 1 and 2 isolates had a lower milk somatic cell count than those infected with isolates of group 3. In addition, Bap-positive isolates were significantly more able to colonize and persist in the bovine mammary gland in vivo and were less susceptible to antibiotic treatments when forming biofilms in vitro. Analysis of the structural bap gene revealed the existence of alternate forms of expression of the Bap protein in S. aureus isolates obtained under field conditions throughout the animal's life. The presence of anti-Bap antibodies in serum samples taken from animals with confirmed S. aureus infections indicated the production of Bap during infection. Furthermore, disruption of the ica operon in a bap-positive strain had no effect on in vitro biofilm formation, a finding which strongly suggested that Bap could compensate for the deficiency of the PIA/PNAG product (a biofilm matrix polysaccharide). Altogether, these results demonstrate that, in the bovine intramammary gland, the presence of Bap may facilitate a biofilm formation connected with the persistence of S. aureus.
机译：金黄色葡萄球菌是引起细菌内感染的常见原因，这种细菌通常会变成慢性细菌，与细菌产生生物膜的能力有关。在这里，我们报告产生慢性牛乳腺炎的能力和生物膜形成之间的关系。我们将牛乳腺炎分类为S。根据生物膜形成所需的特定遗传元件的存在，金黄色葡萄球菌可分为三类：第一类（ ica + bap < sup> + ），第2组（ ica + ， bap 阴性）和第3组（ ica 否， bap 否）。总体而言，自然感染了第1组和第2组分离株的动物比第3组感染的动物具有较低的乳体细胞计数。此外，Bap阳性分离株在体内和体内在牛乳腺中的定居和持久能力明显增强。在体外形成生物膜时，对抗生素治疗的敏感性较低。对 bap 结构基因的分析表明， S中存在Bap蛋白的其他表达形式。在野外条件下获得的金黄色葡萄球菌的分离株。从确诊为 S的动物身上提取的血清样品中存在抗Bap抗体。金黄色葡萄球菌感染表明在感染过程中产生了Bap。此外，破坏 bap 阳性菌株中的 ica 操纵子对体外生物膜的形成没有影响，这一发现强烈表明，Bap可以弥补Bap的不足。 PIA / PNAG产品（生物膜基质多糖）。总而言之，这些结果表明，在牛乳腺中，Bap的存在可能促进与 S持续存在有关的生物膜形成。金黄色。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号