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首页> 外文期刊>Infection and immunity >Specificity of Cryptococcus neoformans factor sera determined by enzyme-linked immunosorbent assay and dot enzyme assay.
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Specificity of Cryptococcus neoformans factor sera determined by enzyme-linked immunosorbent assay and dot enzyme assay.

机译：通过酶联免疫吸附测定和斑点酶测定来确定新隐球菌因子血清的特异性。

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An indirect enzyme-linked immunosorbent assay (ELISA) and a dot enzyme assay (DEA) were used to determine the specificities of Cryptococcus neoformans factor sera to serotype type-specific capsular polysaccharides, glucuronoxylomannans (GXMs). Pure and chemically characterized GXMs were obtained from representative isolates of C. neoformans serotypes A, B, C, and D. Distinctive specificity patterns and quantitative differences were observed for each factor serum when the selected GXMs were studied by ELISA. The specificity patterns for each factor serum determined by DEA almost completely paralleled the ELISA results. The serotype specificities demonstrated by ELISA and DEA were similar to previously reported results that were obtained by slide agglutination studies of whole cells. On the basis of the ELISA and DEA activity patterns, factor sera 5, 6, and 8 were specific for serotypes B, C, and D, respectively; factor serum 1 was strongly reactive to all serotypes; factor serum 2 was specific for serotypes A, B, and D; factor serum 3 was specific for serotypes A and D; and factor serum 4 was specific for serotypes B and C. The specificity of factor serum 7 for serotype A was demonstrated by DEA only. Structural variation was indicated among the serotype C isolates studied because a unique activity pattern versus factor serum 6 was observed for each isolate. The quantitative differences in the activity of the GXMs from five serotype C isolates suggest that mannopyranoside residues substituted O-2 and O-4 with xylose are essential elements of the determinant responsible for the observed activity of factor 6. No significant differences in activity patterns and specificities of factor serum 6 were observed when O-deacetylated GXMs were substituted for the native GXMs. Our results show that ELISA and DEA are valuable techniques for the serological analysis of cryptococcal factor sera and GXMs.
机译：间接酶联免疫吸附测定（ELISA）和点酶测定（DEA）用于确定新隐球菌因子血清对血清型特异性荚膜多糖葡糖醛酸甘露聚糖（GXMs）的特异性。从具有代表性的新孢子虫血清型A，B，C和D的分离株中获得纯的和化学表征的GXM。通过ELISA研究所选GXM时，观察到每种因子血清的特异性特异性模式和定量差异。通过DEA测定的每种因子血清的特异性模式几乎完全与ELISA结果平行。 ELISA和DEA证实的血清型特异性与先前报道的通过全细胞玻片凝集研究获得的结果相似。根据ELISA和DEA活性模式，因子血清5、6和8对B，C和D型血清分别具有特异性。血清因子1对所有血清型均具有强反应性;血清因子2对血清型A，B和D具有特异性;血清因子3对血清型A和D具有特异性;因子血清4对血清型B和C具有特异性。因子血清7对血清型A的特异性仅由DEA证实。在研究的血清型C分离株之间指出了结构变异，因为每个分离株均观察到独特的活性模式与因子血清6的关系。来自五个血清型C分离株的GXMs活性的定量差异表明，用木糖取代O-2和O-4的甘露吡喃糖苷残基是决定因子6观察到活性的决定因素。在活性模式和当O-去乙酰化GXM取代天然GXM时，观察到了因子血清6的特异性。我们的结果表明，ELISA和DEA是对隐球菌因子血清和GXM进行血清学分析的有价值的技术。

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