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首页> 外文期刊>Infection and immunity >Use of pyocin to select a Haemophilus ducreyi variant defective in lipooligosaccharide biosynthesis.
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Use of pyocin to select a Haemophilus ducreyi variant defective in lipooligosaccharide biosynthesis.

机译：霉素用于选择脂寡糖生物合成中有缺陷的杜氏嗜血杆菌变体。

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Haemophilus ducreyi, a cause of genital ulcer disease in developing countries, appears to facilitate the heterosexual transmission of the human immunodeficiency virus in Africa. Despite an increase in studies of this gram-negative human pathogen, little is known about the pathogenesis of chancroid. Our studies have shown that the lipooligosaccharides (LOS) of H. ducreyi may play an important role in ulcer formation. Monoclonal antibody and mass spectrometric analyses identified a terminal trisaccharide present on H. ducreyi LOS that is immunochemically similar to human paragloboside. This epitope is present on the LOS of Neisseria gonorrhoeae, and it may be the site of attachment for pyocin lysis. We have used pyocin, produced by Pseudomonas aeruginosa, to select LOS variants with sequential saccharide deletions from N. gonorrhoeae. On the basis of the similarities between N. gonorrhoeae and H. ducreyi LOS, we employed the same technique to determine if H. ducreyi strains were susceptible to pyocin lysis. In this study, we report the generation of a pyocin N-resistant H. ducreyi strain which synthesizes a truncated version of the parental LOS. Further studies have shown that this H. ducreyi variant has lost the terminal LOS epitope defined by monoclonal antibody 3F11. This report demonstrates that H. ducreyi is sensitive to pyocins and that this technique can be used to generate H. ducreyi LOS variants. Such variants could be used in comparative studies to relate LOS structure to biologic function in the pathogenesis of chancroid.
机译：杜氏嗜血杆菌是发展中国家生殖器溃疡疾病的病因，似乎促进了人类免疫缺陷病毒在非洲的异性传播。尽管对这种革兰氏阴性人类病原体的研究有所增加，但对类虫的发病机理知之甚少。我们的研究表明，杜氏嗜血杆菌的脂寡糖可能在溃疡形成中起重要作用。单克隆抗体和质谱分析确定了在杜氏嗜血杆菌LOS上存在的末端三糖，该末端三糖在免疫化学上类似于人副球蛋白。此表位存在于淋病奈瑟氏球菌的LOS上，它可能是裂解红霉素的附着位点。我们已经使用由铜绿假单胞菌产生的脓素来选择淋病奈瑟氏球菌具有连续糖缺失的LOS变体。基于淋病奈瑟氏球菌和杜克雷氏菌LOS之间的相似性，我们采用了相同的技术来确定杜克雷氏菌菌株是否易于裂解。在这项研究中，我们报告了合成的亲本LOS的截短版本的耐霉素N的H. ducreyi菌株的产生。进一步的研究表明，该杜氏嗜血杆菌变异体已经失去了单克隆抗体3F11定义的末端LOS表位。该报告表明，杜克氏杆菌对pyocins敏感，并且该技术可用于生成杜克氏菌LOS变体。此类变体可用于比较研究中，以将LOS结构与类虫发病机制中的生物学功能联系起来。

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