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首页> 外文期刊>Infection and immunity >Association of Legionella pneumophila with the macrophage endoplasmic reticulum.
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Association of Legionella pneumophila with the macrophage endoplasmic reticulum.

机译：嗜肺军团菌与巨噬细胞内质网的联系。

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摘要

Legionella pneumophila replicates within a membrane-bounded compartment that is studded with ribosomes. In this study we investigated whether these ribosomes originate from the cytoplasmic pool or are associated with host endoplasmic reticulum (ER). Immunofluorescence and electron microscopic localization studies of ER proteins in macrophages infected with L. pneumophila indicated that the bacteria reside in a compartment surrounded by ER. An L. pneumophila mutant that grows slowly in macrophages was slow to associate with host ER, providing genetic evidence in support of the hypothesis that this specialized vacuole is required for intracellular bacterial growth. Ultrastructural studies, in which the ER luminal protein BiP was labeled by immunoperoxidase cytochemistry, revealed that L. pneumophila replication vacuoles resemble nascent autophagosomes. Furthermore, short-term amino acid starvation of macrophages, which stimulated host autophagosomes. Furthermore, short-term amino acid starvation of macrophages, which stimulated host autophagy, increased association of the bacteria with the ER and enhanced bacterial growth. These results are compatible with the hypothesis that L. pneumophila exploits the autophagy machinery of macrophages to establish an intracellular niche favorable for replication.
机译：嗜肺军团菌在膜核定的隔室中复制，该室中布满了核糖体。在这项研究中，我们调查了这些核糖体是否起源于细胞质池或与宿主内质网（ER）相关。肺炎链球菌感染的巨噬细胞中ER蛋白的免疫荧光和电子显微镜定位研究表明，细菌位于ER包围的隔室内。在巨噬细胞中生长缓慢的嗜肺乳杆菌突变体与宿主ER的结合较慢，提供了遗传学证据来支持这种特殊的液泡是细胞内细菌生长所必需的假说。通过免疫过氧化物酶细胞化学标记ER腔蛋白BiP的超微结构研究表明，嗜肺乳杆菌复制液泡类似于新生的自噬体。此外，巨噬细胞的短期氨基酸饥饿会刺激宿主自噬体。此外，巨噬细胞的短期氨基酸饥饿会刺激宿主自噬，增加细菌与内质网的结合并增强细菌的生长。这些结果与假单胞菌利用巨噬细胞的自噬机制建立有利于复制的细胞内利基的假说相符。

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