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首页> 外文期刊>Infection and immunity >Candida albicans cell walls contain the fluorescent cross-linking amino acid dityrosine.
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Candida albicans cell walls contain the fluorescent cross-linking amino acid dityrosine.

机译：白色念珠菌细胞壁含有荧光交联氨基酸二酪氨酸。

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Several clinical and laboratory isolates of Candida albicans have a natural blue surface fluorescence when cultured and observed with sensitive optics. The localization and color of the fluorescence are similar to those of the natural fluorescence of sporulated Saccharomyces cerevisiae which is caused by the generation and surface deposition of the cross-linking amino acid dityrosine. In S. cerevisiae, dityrosine production results from the direct action of at least two genes and is responsible for resistance of the ascospores to lytic enzymes and physicochemical trauma. Among the criteria for the identification of dityrosine is pH sensitivity of the fluorescence intensity and a highly characteristic shift of the fluorescence excitation maximum with a change in pH. Video microscopy of whole Candida organisms revealed the characteristic dityrosine intensity maximum at pH approximately 10 and the intensity minimum at pH approximately 2. Separation of an acid hydrolysate of Candida cell walls by reverse-phase high-performance liquid chromatography revealed a fluorescence peak that coelutes with the reagent dityrosine. At pH approximately 10, this peak has a fluorescence excitation maximum of 320 to 325 nm, while at pH approximately 2, the excitation maximum is 285 to 290 nm. This excitation maximum shift and the observed emission maximum of approximately 410 nm are characteristic of dityrosine. Two separate strains of C. albicans were injected intraperitoneally into mice and harvested at 24 h. Blue surface fluorescence was observed, suggesting that dityrosine generation occurs in vivo as well as in vitro. This is the first report of the presence of dityrosine in a human fungal pathogen.
机译：培养和观察敏感光学时，白色念珠菌的几种临床和实验室分离株均具有天然的蓝色表面荧光。荧光的定位和颜色类似于孢子状酿酒酵母的天然荧光，其是由交联氨基酸二酪氨酸的产生和表面沉积引起的。在酿酒酵母中，二氢酪氨酸的产生是由至少两个基因的直接作用引起的，并引起了子囊孢子对分解酶和理化损伤的抗性。鉴定二氢酪氨酸的标准包括荧光强度的pH敏感性和随pH值变化的最大荧光激发的高度特征性变化。整个念珠菌生物体的视频显微镜显示，在大约pH值为10时，特征性二氢尿嘧啶强度最大，在大约pH值为2时，强度最小。通过反相高效液相色谱分离假丝酵母细胞壁的酸性水解产物，发现荧光峰与试剂二酪氨酸。在pH约为10时，此峰的荧光激发最大值为320至325 nm，而在pH约为2时，激发最大值为285至290 nm。该激发最大位移和观察到的约410nm的最大发射是二酪氨酸的特征。将两个单独的白色念珠菌菌株腹膜内注射到小鼠中，并在24 h收获。观察到蓝色表面荧光，表明在体内以及体外都发生二氢鸟氨酸的产生。这是人类真菌病原体中存在二氢酪氨酸的首次报道。

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