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首页> 外文期刊>Infection and immunity >Iron-repressible outer membrane proteins of Helicobacter pylori involved in heme uptake.
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Iron-repressible outer membrane proteins of Helicobacter pylori involved in heme uptake.

机译：幽门螺杆菌的铁抑制性外膜蛋白参与血红素的吸收。

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摘要

Helicobacter pylori is known to be a causative agent of gastritis and peptic ulcer disease in humans. The acquisition of iron from the human host may contribute greatly to the virulence of this organism. To study this, H. pylori was cultured under iron-restrictive conditions to induce synthesis of possible iron-regulated outer membrane proteins. This was achieved by the addition of 20% (vol/vol) heat-inactivated newborn calf serum, which contains iron-binding proteins like transferrin and albumin, and no free iron. The newborn calf serum was able to bind free ionic iron in brucella broth culture medium. Electrophoretic analysis of outer membrane preparations from H. pylori cultured under conditions of iron restriction showed several proteins to be present at elevated levels. These appeared to be iron-repressible outer membrane proteins (IROMPs). In addition, IROMPs with molecular sizes of 77, 50, and 48 kDa were isolated by use of hemin-agarose affinity chromatography. These three heme-binding IROMPs might be involved in the uptake of heme from the host and might therefore be important virulence factors of H. pylori.
机译：幽门螺杆菌是人类胃炎和消化性溃疡疾病的病原体。从人类宿主中获取铁可能极大地提高了这种生物的毒性。为了研究这一点，在铁限制条件下培养幽门螺杆菌，以诱导可能的铁调节的外膜蛋白的合成。这是通过添加20％（体积/体积）热灭活的新生小牛血清来实现的，该血清含有铁结合蛋白（如转铁蛋白和白蛋白），并且不含游离铁。新生小牛血清能够结合布鲁氏菌肉汤培养基中的游离离子铁。在铁限制条件下培养的幽门螺杆菌的外膜制剂的电泳分析表明，几种蛋白质以升高的水平存在。这些似乎是铁可抑制的外膜蛋白（IROMPs）。另外，通过使用血红素-琼脂糖亲和层析分离了具有77、50和48kDa的分子大小的IROMP。这三个与血红素结合的IROMP可能参与宿主对血红素的摄取，因此可能是幽门螺杆菌的重要毒力因子。

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