...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Borrelia burgdorferi vesicle production occurs via a mechanism independent of immunoglobulin M involvement.
【24h】

Borrelia burgdorferi vesicle production occurs via a mechanism independent of immunoglobulin M involvement.

机译：伯氏疏螺旋体小泡的产生通过独立于免疫球蛋白M参与的机制发生。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Borrelia burgdorferi produces extracellular vesicles containing various borrelial protein antigens when propagated in vitro in culture media. Commonly observed components of borrelial vesicle preparations are borrelial surface antigens, bovine serum albumin, and the heavy chains of rabbit immunoglobulin G and immunoglobulin M. This study employed ultracentrifugation to harvest borrelial vesicles and analyzed these preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblotting. We demonstrated that the rabbit mu heavy-chain band observed was devoid of OspA or at most levels below those detectable by immunoblot. We also demonstrated the recovery of borrelial vesicles at relative centrifugal forces as low as 25,000 x g, compared with the force of > 200,000 x g normally employed. Further, the mu heavy-chain band was recovered from uninoculated growth media processed at 25,000 x g, suggesting that it behaves as a particle rather than as a soluble molecule under these conditions. Lastly, vesicles were demonstrated to be present in preparations harvested from growth media supplemented with fetal calf serum, suggesting that vesicle production by B. burgdorferi can occur in the absence of immunoglobulins.
机译：当在培养基中体外繁殖时，伯氏疏螺旋体产生含有多种疏螺旋体蛋白抗原的细胞外囊泡。常见的含硼小泡制剂的成分为含硼的表面抗原，牛血清白蛋白以及兔免疫球蛋白G和免疫球蛋白M的重链。本研究采用超速离心法收集了含小泡的囊泡，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和Western分析了这些制剂免疫印迹。我们证明观察到的兔μ重链条带没有OspA或至多低于免疫印迹可检测到的水平。我们还证明了在相对低至25,000 x g的相对离心力下，相对于通常使用的> 200,000 x g的力，回收了小囊泡。此外，从以25,000 x g处理的未接种生长培养基中回收到mu重链带，这表明在这些条件下它的行为像颗粒而不是可溶性分子。最后，从补充有胎牛血清的生长培养基中收获的制剂中证实存在囊泡，这表明B. burgdorferi的囊泡生产可能在没有免疫球蛋白的情况下发生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号