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首页> 外文期刊>Infection and immunity >The V-region repertoire of Haemophilus influenzae type b polysaccharide antibodies induced by immunization of infants.
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The V-region repertoire of Haemophilus influenzae type b polysaccharide antibodies induced by immunization of infants.

机译：通过婴儿免疫诱导的流感嗜血杆菌b型多糖抗体的V区组成。

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Haemophilus influenzae type b (Hib) is a significant pathogen for young children, and three Hib vaccines (named PRP-OMPC, HbOC, and PRP-T) are currently available for young children. Extensive studies of anti-Hib polysaccharide (PS) antibodies (Abs) have shown that the V regions of Abs against the Hib PS comprise a VH gene in the VH3 gene family and a VL gene from various K kappa and V lambda subgroups. To study immunogenic properties of the three vaccines in young children, we determined the VL subgroups and avidities of anti-Hib-PS Abs induced by the three clinically available conjugate vaccines. Ab avidity was measured by determining the concentration of a Hib-PS oligomer that abrogates half of the binding of immunoglobulin G anti-Hib-PS Abs to microwells. The PRP-OMPC vaccine induced lower-avidity Abs than the prelicensure HbOC vaccine (P = 0.05). When we compared anti-Hib-PS Abs expressing V kappa Ia, V kappa II, and V lambda subgroups, a greater Ab response was induced by the prelicensure HbOC vaccine than other vaccines (P < 0.05). When anti-Hib-PS Abs with the V kappa III subgroup were compared, however, both PRP-T and prelicensure HbOC vaccines induced a comparable response, which in turn was greater than those induced by the PRP-OMPC or the postlicensure HbOC vaccine (P < 0.001). The VL repertoire of Abs induced with the prelicensure HbOC or PRP-T vaccine in young children is dominated (about 80%) by anti-Hib-PS Abs using subgroup V kappa II. However, anti-Hib-PS using V kappa II VL accounts for only about 40% of the total anti-Hib-PS Abs induced with the PRP-OMPC vaccine or the postlicensure HbOC. Our data suggest that immunogenic properties of Hib vaccines in young children vary depending on the vaccine preparations as well as the vaccine types.
机译：b型流感嗜血杆菌（Hib）是幼儿的重要病原体，目前有3种Hib疫苗（分别称为PRP-OMPC，HbOC和PRP-T）可供儿童使用。抗-Hib多糖（PS）抗体（Abs）的广泛研究表明，针对Hib PS的Abs的V区包含VH3基因家族中的VH基因以及来自各种Kκ和V lambda亚组的VL基因。为了研究这三种疫苗在幼儿中的免疫原性，我们确定了由三种临床上可用的结合疫苗诱导的抗Hib-PS Ab的VL亚型和亲和力。通过确定消除免疫球蛋白G抗Hib-PS Ab与微孔结合一半的Hib-PS低聚物的浓度来测量抗体亲和力。 PRP-OMPC疫苗诱导的Abs低于许可前的HbOC疫苗（P = 0.05）。当我们比较表达V kappa Ia，V kappa II和V lambda亚型的抗Hib-PS Abs时，预许可HbOC疫苗诱导的Ab反应大于其他疫苗（P <0.05）。但是，当比较具有V kappa III亚型的抗Hib-PS Abs时，PRP-T和许可前HbOC疫苗均诱导了类似的反应，反过来又大于PRP-OMPC或许可后HbOC疫苗诱导的反应（ P <0.001）。使用Hk-PS亚组V的抗Hib-PS Abs可以控制（约80％）预先接种HbOC或PRP-T疫苗诱导的Abs的VL抗体库。但是，使用V kappa II VL的抗Hib-PS仅占PRP-OMPC疫苗或许可后HbOC诱导的抗Hib-PS抗体总数的40％。我们的数据表明，Hib疫苗在幼儿中的免疫原性取决于疫苗制剂和疫苗类型。

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