• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Changes in the peripheral blood T-Cell receptor V beta repertoire in vivo and in vitro during shigellosis.
【24h】

Changes in the peripheral blood T-Cell receptor V beta repertoire in vivo and in vitro during shigellosis.

机译：志贺菌病期间体内和体外外周血T细胞受体V beta的变化。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A sequential activation of T cells in peripheral blood during shigello sis has been observer (D. Islam, P.K. Bradham, A. A. Lindberg, and B. Christensson, Infect. Immun 63:2941-2949, 1995). To further investigate the cellular response during the course of Shigella infection, changes in the T-cell receptor (TCR) repertoire in the subsets in blood in patients during shigellosis was that Shigella antigens may modulate the function of T cells carrying TCRs capable of recognizing Shigella-specific epitopes or superantigens. Such a selective preference for T cells expressing certain TCR Vbeta types could lead to the expansion or deletion of these T cells. In the present study of 27 adult male Bangladeshi patients with dysentery (14 cases caused by Shigella Dysenteriae 1 and 13 cases caused by Shigella flexneri), the changes in the TCR Vbeta repertoire of peripheral blood CD4+ and CD8+ T-cell subsets have been analyzed with a panel of nine anti-Vbeta monoclonal antibodies by flow cytometry. Twenty healthy males from Bangladesh and 20 healthy males from Sweden served as controls. Compared with the Bangladeshi controls, the patients had an increased frequency of CD4+T cells expression Vbeta2, Vbeta3, and Vbeta17, with a maximum at day 7 after the onset of disease. The frequency of CD4+T cells expressing Vbeta5.1 was increased only in patients with S. flexneri infection. Peripheral blood T cells from Shigella-infected patients also responded to in vitro stimulation in a TCR Vbeta-specific manner. Stimulation with heat-killed S. dysenteriae 1 and Shiga toxin enhanced the frequency of cells expressing Vbeta2, Vbeta3, Vbeta5.1, Vbeta13.6, and Vbeta17, especially in samples obtained at day 7. The enhanced frequency of cells expressing Vbeta2, Vbeta3, Vbeta5.1, and Vbeta17 found both in in vivo and in vitro could suggest that in shigellosis antigens or superantigens are presented to the immune system and preferentially activate certain TCR Vbeta types in T-cell subsets. The kinetics of the change in the TCR Vbeta repertoire in blood during shigellosis may indicate that following local activation, the antigen activated T cells can be retrieved in the blood and restimulated in vitro. If confirmed by parallel analysis of T cells in the gut and blood by TCR sequence analysis, the possibility suggested by our findings would facilitate further analysis of the role of cell-mediated immune responses in the pathogenesis of and protection against Shigella infection.
机译：在志贺氏菌病期间外周血中T细胞的顺序活化是观察者（D.Islam，P.K。Bradham，A.A.Lindberg，和B.Christensson，Infect.Immun 63：2941-2949，1995）。为了进一步研究志贺氏菌感染过程中的细胞反应，志贺氏菌病患者血液亚群中T细胞受体（TCR）组成的变化是志贺氏菌抗原可能会调节携带能够识别志贺氏菌的TCR的T细胞的功能特异性表位或超抗原。对表达某些TCR Vbeta类型的T细胞的这种选择性偏爱可能导致这些T细胞的扩增或缺失。在本研究中，对27名孟加拉国成年男性痢疾患者（由痢疾志贺氏菌引起的14例，由弗氏志贺氏菌引起的13例），分析了外周血CD4 +和CD8 + T细胞亚群的TCR Vbeta组成变化。流式细胞术检测一组九种抗Vbeta单克隆抗体。孟加拉国20名健康男性和瑞典20名健康男性作为对照。与孟加拉国对照组相比，患者的CD4 + T细胞表达Vbeta2，Vbeta3和Vbeta17的频率增加，在发病后第7天达到最高。仅在患有弗氏链球菌感染的患者中，表达Vbeta5.1的CD4 + T细胞的频率增加。志贺氏菌感染患者的外周血T细胞也以TCR Vbeta特异性方式响应体外刺激。用热杀死的痢疾链球菌1和志贺毒素刺激，可以提高表达Vbeta2，Vbeta3，Vbeta5.1，Vbeta13.6和Vbeta17的细胞的频率，尤其是在第7天获得的样品中。表达Vbeta2，Vbeta3的细胞的频率增加。 ，体内和体外发现的Vbeta5.1和Vbeta17可能表明，在志贺菌病中，抗原或超抗原被呈递给免疫系统，并优先激活T细胞亚群中某些TCR Vbeta类型。志贺菌病期间血液中TCR Vbeta组成变化的动力学可能表明，局部活化后，抗原活化的T细胞可以在血液中回收并在体外重新刺激。如果通过TCR序列分析对肠道和血液中T细胞进行平行分析得到证实，我们发现的可能性将有助于进一步分析细胞介导的免疫应答在志贺氏菌感染的发病机理和预防中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号