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首页> 外文期刊>Infection and immunity >Invasion of dentinal tubules by oral streptococci is associated with collagen recognition mediated by the antigen I/II family of polypeptides.
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Invasion of dentinal tubules by oral streptococci is associated with collagen recognition mediated by the antigen I/II family of polypeptides.

机译：口腔链球菌对牙本质小管的侵袭与多肽的抗原I / II家族介导的胶原识别有关。

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Cell surface proteins SspA and SspB in Streptococcus gordonii and SpaP in Streptococcus mutans are members of the antigen I/II family of polypeptides produced by oral streptococci. These proteins are adhesins and mediate species-specific binding of cells to a variety of host and bacterial receptors. Here we show that antigen I/II polypeptides are involved in the attachment of oral streptococci to collagen and that they also determine the ability of these bacteria to invade human root dentinal tubules. Wild-type S. gordonii DL1 (Challis) cells showed heavy invasion of tubules to a depth of approximately 200 microm, whereas the abilities of cells of isogenic mutant strains OB220 (sspA) and OB219 (sspA sspB) to invade were 50 and >90% reduced, respectively. Likewise, wild-type S. mutans NG8 cells invaded dentinal tubules, whereas cells of isogenic mutant strain 834 (spaP) did not. The invasive abilities of strains OB220 and OB219 were restored by heterologous expression of S. mutans SpaP polypeptide in these strains. The extents of tubule invasion by various wild-type and mutant strains correlated with their levels of adhesion to type I collagen, a major component of dentin. Furthermore, S. gordonii DL1 cells exhibited a growth response to collagen by forming long chains. This was not shown by ssp mutants but was restored by the expression of SpaP in these cells. The production of SspA polypeptide by S. gordonii DL1, but not production of SspB polypeptide by strain OB220 (sspA), was enhanced in the presence of collagen. These results are the first to demonstrate that antigen I/II family polypeptides bind collagen and mediate a morphological growth response of streptococci to collagen. These antigen I/II polypeptide activities are critical for intratubular growth of streptococci and thus for establishment of endodontic infections.
机译：戈登链球菌中的细胞表面蛋白SspA和SspB和变形链球菌中的SpaP是口服链球菌产生的多肽抗原I / II家族的成员。这些蛋白质是粘附素，可介导细胞与多种宿主和细菌受体的物种特异性结合。在这里，我们显示抗原I / II多肽参与口腔链球菌与胶原蛋白的结合，并且它们还决定了这些细菌入侵人牙根小管的能力。野生型戈登氏酵母DL1（Challis）细胞显示出严重侵袭小管，深度约为200微米，而同基因突变株OB220（sspA）和OB219（sspA sspB）的细胞入侵能力为50和> 90减少了％。同样，野生型变形链球菌NG8细胞侵入牙本质小管，而同基因突变菌株834（spaP）则没有。通过变形链球菌SpaP多肽在这些菌株中的异源表达，恢复了菌株OB220和OB219的侵袭能力。各种野生型和突变型菌株侵袭小管的程度与其对I型胶原（牙本质的主要成分）的粘附水平有关。此外，戈登氏链球菌DL1细胞通过形成长链表现出对胶原蛋白的生长反应。 ssp突变体未显示出这种现象，但通过这些细胞中SpaP的表达得以恢复。在胶原蛋白的存在下，戈登氏酵母DL1产生SspA多肽，而OB220菌株（sspA）没有产生SspB多肽。这些结果首次证明抗原I / II家族多肽结合胶原并介导链球菌对胶原的形态生长反应。这些抗原I / II多肽活性对于链球菌的肾小管内生长并因此对牙髓感染的建立至关重要。

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