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首页> 外文期刊>Infection and immunity >Secretory Phospholipase A2 Is the Principal Bactericide for Staphylococci and Other Gram-Positive Bacteria in Human Tears
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Secretory Phospholipase A2 Is the Principal Bactericide for Staphylococci and Other Gram-Positive Bacteria in Human Tears

机译：分泌型磷脂酶A2是人类眼泪中葡萄球菌和其他革兰氏阳性细菌的主要杀菌剂

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摘要

We examined human tears for molecules that killed gram-positive bacteria. The principal mediator of bactericidal activity against staphylococci proved to be a calcium-dependent enzyme, secretory phospholipase A2. Whereas the concentration of secretory phospholipase A2 in the normal tear film exceeded 30 μg/ml, only 1.1 ng (<0.1 nM) of the enzyme per ml sufficed to killListeria monocytogenes and 15 to 80 ng/ml killedStaphylococcus aureus. Despite its efficacy against gram-positive bacteria, secretory phospholipase A2 lacked bactericidal activity against gram-negative organisms (Escherichia coli, Salmonella typhimurium, andPseudomonas aeruginosa) when tested in the ionic environment of tears. Given the presence of secretory phospholipase A2 in tears, intestinal secretions, and leukocytes, this enzyme may play a substantial role in innate mucosal and systemic bactericidal defenses against gram-positive bacteria.
机译：我们检查了人的眼泪中是否有杀死革兰氏阳性细菌的分子。事实证明，针对葡萄球菌的杀菌活性的主要介质是钙依赖性酶，即分泌型磷脂酶A 2 。正常泪膜中分泌性磷脂酶A 2 的浓度超过30μg/ ml，每毫升仅1.1 ng（<0.1 nM）的酶足以杀死单核细胞增生性李斯特菌并杀死15至80 ng / ml的金黄色葡萄球菌。尽管分泌型磷脂酶A 2 具有抗革兰氏阳性菌的功效，但它对革兰氏阴性菌（大肠杆菌，鼠伤寒沙门氏菌和铜绿假单胞菌）在眼泪的离子环境中进行测试。考虑到眼泪，肠道分泌物和白细胞中存在分泌性磷脂酶A 2 ，该酶可能在针对革兰氏阳性细菌的固有粘膜和全身杀菌防御中发挥重要作用。

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