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首页> 外文期刊>Infection and immunity >Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine.
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Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine.

机译：在C3H / HeJ和C3H / HeNCrlBR小鼠中感染鼠伤寒沙门氏菌的免疫力：用芳香族依赖性活鼠伤寒沙门氏菌菌株作为疫苗的研究。

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Immunization with avirulent Salmonella typhimurium strain SL3235, a smooth, aroA- derivative, was shown to induce high levels of resistance to challenge with virulent S. typhimurium in innately hypersusceptible C3H/HeJ mice and inherently resistant C3H/HeNCrlBR mice. Strain SL3235 is one of a class of avirulent aroA- derivatives made from various strains and species of Salmonella that are being considered as vaccine candidates for cattle and humans. This paper supports their efficacy and potential utility in this regard. In C3H/HeJ mice, immunity against over 1,000 50% lethal doses of virulent S. typhimurium was evident as early as 3 days after immunization and persisted for at least 7 months. Further, the vaccine was effective over a broad spectrum of doses, ranging from 10(4) to 10(6) organisms. Infection with SL3235 led to marked splenomegaly in both mouse strains. The relationship of splenomegaly to the growth kinetics and colonization by SL3235 in the spleens of infected C3H/HeJ and C3H/HeNCrlBR mice was followed. SL3235 initially multiplied slowly in the spleens of both mouse strains and then was rapidly cleared. Less multiplication was seen in the resistant C3H/HeNCrlBR mice than in C3H/HeJ mice. Maximum splenomegaly occurred after clearance of the organism had begun. Protection against virulent S. typhimurium persisted after virtually all of the SL3235 vaccine strain had been cleared from the spleen. Cross-protection against Listeria monocytogenes was evident, but had a later onset, waned by 21 days, and was not detectable by 1 month after vaccination. Demonstration of this cross-protection is consistent with the interpretation that SL3235 induces cellular immunity. One-week immune spleen cells adoptively transferred anti-S. typhimurium and anti-L. monocytogenes immunity. T cell-enriched fractions were ineffective in adoptive transfer, as were spleen cells taken 2 weeks or later after immunization. Protective capacity was in the adherent cell fraction and seemed to be associated with macrophages. Evidence for induction of a population of sensitized T cells was obtained by using a peritoneal exudate T-lymphocyte proliferation assay on peritoneal T lymphocytes collected 1 to 3 months after SL3235 infection.
机译：在先天性高敏感性C3H / HeJ小鼠和固有耐药性C3H / HeNCrlBR小鼠中，用无毒的鼠伤寒沙门氏菌菌株SL3235（一种光滑的aroA衍生物）进行免疫接种可诱导高水平抵抗强毒鼠伤寒沙门氏菌的攻击。 SL3235菌株是由各种沙门氏菌菌株和物种制成的无毒aroA衍生物中的一种，被认为是牛和人的候选疫苗。本文在这方面支持它们的功效和潜在用途。在C3H / HeJ小鼠中，最早在免疫后3天就对超过1,000个50％致死剂量的鼠伤寒沙门氏菌产生了免疫力，并持续了至少7个月。此外，该疫苗在从10（4）到10（6）个生物体的广泛剂量范围内均有效。 SL3235感染在两种小鼠品系中均导致明显的脾肿大。追踪脾脏肿大与感染的C3H / HeJ和C3H / HeNCrlBR小鼠脾脏中SL3235的生长动力学和定殖的关系。 SL3235最初在两种小鼠品系的脾脏中缓慢繁殖，然后迅速清除。在抗性C3H / HeNCrlBR小鼠中观察到的增殖比在C3H / HeJ小鼠中少。开始清除生物体后出现最大的脾肿大。实际上，从脾脏清除了所有SL3235疫苗株后，针对强毒鼠伤寒沙门氏菌的保护仍然有效。对单核细胞增生性李斯特菌的交叉保护是明显的，但起病较晚，在21天后减弱，并且在接种疫苗后1个月无法检测到。这种交叉保护的证明与SL3235诱导细胞免疫的解释一致。一周的免疫脾细胞过继转移了抗S。鼠伤寒和抗L。单核细胞增生性免疫。富含T细胞的级分在过继转移方面无效，免疫后2周或更晚服用的脾细胞也是如此。保护能力在粘附细胞部分，似乎与巨噬细胞有关。通过对SL3235感染后1到3个月收集的腹膜T淋巴细胞进行腹膜渗出液T淋巴细胞增殖试验，获得了诱导敏化T细胞群体的证据。

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