• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Immunochemical characterization of P pili from invasive Escherichia coli.
【24h】

Immunochemical characterization of P pili from invasive Escherichia coli.

机译：侵袭性大肠杆菌的菌毛菌毛的免疫化学表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

P pili (or fimbriae) are present on most pyelonephritogenic Escherichia coli strains, and they mediate binding to erythrocytes and epithelial cells. To determine the antigenic diversity of P pili, we purified the pili from 14 bacteremic E. coli strains which caused mannose-resistant hemagglutination. Pilus preparations consisted of one to three bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ranged in molecular weight from 14,000 to 19,500. There was no single band common to all the strains. An enzyme-linked immunosorbent inhibition assay detected 20 ng of pilus antigen. When four different rabbit antisera were used, only two or fewer heterologous strains could inhibit the enzyme-linked immunosorbent assay. Immunoblots yielded the same results. Protein sequences of four P pili had identical N termini. These results show that despite having identical amino-terminal sequences, P pili are antigenically heterogeneous. The receptor-binding domains which are likely to be identical in all strains must be immunorecessive.
机译：P菌毛（或菌毛）存在于大多数产生丙酮酸的大肠杆菌菌株中，并且它们介导与红细胞和上皮细胞的结合。为了确定P菌毛的抗原多样性，我们从引起甘露糖抗性血凝的14种细菌性大肠杆菌菌株中纯化了菌毛。 Pilus制剂由十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的1至3条带组成，分子量范围为14,000至19,500。没有所有菌株共有的单一条带。酶联免疫吸附抑制试验检测到20 ng菌毛抗原。当使用四种不同的兔抗血清时，只有两个或更少的异源菌株可以抑制酶联免疫吸附测定。免疫印迹得到相同的结果。四个P菌毛的蛋白质序列具有相同的N末端。这些结果表明，尽管P pili具有相同的氨基末端序列，但在抗原上是异质的。在所有菌株中可能相同的受体结合域必须是免疫隐性的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号