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首页> 外文期刊>Infection and immunity >Mimicry of human histocompatibility HLA-B27 antigens by Klebsiella pneumoniae.
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Mimicry of human histocompatibility HLA-B27 antigens by Klebsiella pneumoniae.

机译：肺炎克雷伯菌模拟人类组织相容性HLA-B27抗原。

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Anti-HLA-B27 monoclonal antibody M2, which was relatively specific for human histocompatibility antigen HLA-B27, was used to test several bacteria, some of which could potentially induce chronic arthritis in HLA-B27-positive individuals. Using the Western blot procedure, we observed positive reactions with 80,000- and 60,000-dalton antigens with one strain of Klebsiella pneumoniae. Reactivity was not observed with five other monoclonal antibodies which were not reactive with HLA-B27 antigens, nor was reactivity observed with seven other gram-negative bacteria, irrespective of their arthritis-causing potential. To test the validity of our observation, the 80,000-dalton Klebsiella cross-reactive antigen was isolated and used to generate an immune guinea pig serum. We found that the reactivity of this guinea pig serum with Klebsiella envelopes in an enzyme-linked immunosorbent assay was adversely affected by absorption with HLA-B27-positive cells. Our results support the existence of mimicry between HLA-B27 antigens and bacteria.
机译：对人类组织相容性抗原HLA-B27相对特异性的抗HLA-B27单克隆抗体M2用于测试几种细菌，其中一些细菌可能在HLA-B27阳性个体中诱发慢性关节炎。使用蛋白质印迹法，我们观察到80,000和60,000道尔顿抗原与一株肺炎克雷伯菌的阳性反应。与其他五种与HLA-B27抗原不具有反应性的单克隆抗体均未观察到反应性，也与其他七种革兰氏阴性细菌均未观察到反应性，无论它们是否具有引起关节炎的潜力。为了检验我们观察的有效性，分离了80,000道尔顿的克雷伯氏菌交叉反应抗原，并将其用于产生免疫豚鼠血清。我们发现在HLA-B27阳性细胞吸收后，这种豚鼠血清与克雷伯菌包膜的反应性在酶联免疫吸附测定中受到不利影响。我们的结果支持HLA-B27抗原和细菌之间存在拟态。

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