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Strain-specific antigens of Toxoplasma gondii.

机译：弓形虫的菌株特异性抗原。

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A detailed immunological assessment of strain-specific antigens of Toxoplasma gondii has not been reported. We developed rabbit antisera against three strains of toxoplasma obtained from divergent sources. These strains included the frequently studied laboratory strain RH, strain C, obtained from a naturally infected kitten, and strain P, which is maintained by passage in mice. The rabbit antisera were used to identify unique strain-specific and commonly shared tachyzoite antigens by radioiodination followed by immunoprecipitation and Western blot analysis. Both qualitative and quantitative differences of a number of the major tachyzoite antigens were found in these assays. A parasite plaque reduction assay using parasiticidal monoclonal antibody showed marked differences in the ability to kill these three different tachyzoite subtypes, further supporting antigenic variation among T. gondii strains.
机译：弓形虫的菌株特异性抗原的详细免疫学评估尚未见报道。我们开发了针对从不同来源获得的三种弓形虫菌株的兔抗血清。这些菌株包括经常研究的实验室菌株RH，从自然感染的小猫获得的C菌株和通过在小鼠中传代而维持的P菌株。兔抗血清用于通过放射碘化，免疫沉淀和蛋白质印迹分析来鉴定独特的菌株特异性和常见的速殖子抗原。在这些测定中发现了许多主要速殖子抗原的定性和定量差异。使用杀寄生虫单克隆抗体进行的寄生虫斑块减少试验显示，杀死这三种不同的速殖子亚型的能力存在显着差异，进一步支持弓形虫菌株之间的抗原变异。

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