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首页> 外文期刊>Infection and immunity >Stimulation of human polymorphonuclear leukocyte oxidative metabolism by type 1 pili from Escherichia coli.
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Stimulation of human polymorphonuclear leukocyte oxidative metabolism by type 1 pili from Escherichia coli.

机译：大肠杆菌1型菌毛刺激人多形核白细胞氧化代谢。

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We compared the degree to which Escherichia coli phase variants which do (T1P+ E. coli) or do not (T1P- E. coli) express type 1 pili (T1P) stimulate human polymorphonuclear leukocyte (PMN) oxidative activity. Unopsonized T1P+ E. coli stimulated the release of 0.20 to 0.24 nmol of H2O2 per 10(6) PMN per min and the consumption of 1.4 to 4.0 nmol of O2 per 10(6) PMN per min; no measurable PMN oxidative activity was stimulated by unopsonized T1P- E. coli. In the presence of serum opsonins, T1P+ E. coli stimulated the release of 1.12 to 1.16 nmol of H2O2 per 10(6) PMN per min and the consumption of 5.0 to 6.0 nmol of O2 per 10(6) PMN per min, whereas T1P- E. coli stimulated the release of 0.42 to 0.43 nmol of H2O2 per 10(6) PMN per min and the consumption of 0.6 to 2.0 nmol of O2 per 10(6) PMN per min. Although unaggregated T1P did not stimulate PMN, latex beads coated with T1P (T1P-latex) stimulated alpha-methylmannoside-inhibitable, opsonin-independent PMN oxidative activity. The activity stimulated by either T1P+ E. coli or T1P-latex was susceptible to inhibition by cytochalasin B. Latex particles coated with bovine serum albumin or mannose-resistant pili did not stimulate PMN. These data indicate that T1P+ E. coli stimulate PMN oxidative metabolism more effectively than do T1P- E. coli and that a similar PMN oxidative response follows cellular stimulation by either unopsonized T1P+ or opsonized T1P- E. coli. Furthermore, T1P-latex faithfully mimics the ability of T1P+ E. coli to stimulate PMN oxidative metabolism. Such particles may be useful in further analyses of cellular responses to T1P+ E. coli.
机译：我们比较了表达（1类+大肠杆菌）或不表达（1类-大肠杆菌）（1类菌毛）的大肠杆菌相变体刺激人多形核白细胞（PMN）氧化活性的程度。未调理过的T1P +大肠杆菌每10（6）PMN每分钟刺激释放0.20至0.24 nmol的H2O2，每10（6）PMN每分钟刺激释放1.4至4.0 nmol的氧气;未调理过的T1P-大肠杆菌不会刺激可测量的PMN氧化活性。在存在血清调理素的情况下，T1P +大肠杆菌每10（6）PMN每分钟刺激释放1.12至1.16 nmol的H2O2，每分钟10（6）PMN刺激5.0至6.0 nmol的氧气的释放，而T1P -大肠杆菌刺激每10（6）PMN每分钟释放0.42至0.43 nmol的H2O2，每分钟10（6）PMN释放0.6至2.0 nmol的氧气。尽管未聚集的T1P不会刺激PMN，但涂有T1P的乳胶珠（T1P-乳胶）可刺激α-甲基甘露糖苷抑制，调理素独立的PMN氧化活性。 T1P +大肠杆菌或T1P乳胶刺激的活性容易受到细胞松弛素B的抑制。涂有牛血清白蛋白或抗甘露糖菌毛的乳胶颗粒不会刺激PMN。这些数据表明，T1P +大肠杆菌比T1P-大肠杆菌更有效地刺激PMN氧化代谢，并且类似的PMN氧化反应是由未调理过的T1P +或调理过的T1P-大肠杆菌引起的。此外，T1P乳胶忠实地模仿T1P +大肠杆菌刺激PMN氧化代谢的能力。这样的颗粒可用于进一步分析对T1P +大肠杆菌的细胞应答。

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