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首页> 外文期刊>Infection and immunity >Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum.
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Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum.

机译：荚膜组织胞浆自发无毒变体的分离和鉴定。

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A selection procedure was developed which allowed us to isolate spontaneous isogenic avirulent clones from virulent strains of Histoplasma capsulatum. The avirulent yeasts had a unique phenotype: they did not aggregate like the parental strains but grew as dispersed budded and unbudded single cells in liquid medium. On solid medium, the avirulent variant strains grew as smooth-textured colonies, whereas the virulent parental strains grew as rough convoluted colonies. Virulence testing in mice demonstrated that the smooth variants gave 50% lethal dose values similar to those of the avirulent Downs strain. Growth curves for the paired rough and smooth strains were similar. Furthermore, they had the same protein profiles when crude cell fractions were separated on one-dimensional polyacrylamide gels or when whole-cell extracts were separated by two-dimensional gel electrophoresis. Electrophoresis of culture supernatants, however, revealed a difference in a released low-molecular-weight peptide that may be related to virulence. In addition to their usefulness in comparative virulence studies, these avirulent strains should prove valuable for H. capsulatum genetic experiments because of the unique ability of these yeasts to grow without clumping.
机译：开发了选择程序，其使我们能够从荚膜组织胞浆菌的强毒株中分离自发的等基因无毒克隆。无毒酵母具有独特的表型：它们不像亲本菌株那样聚集，而是在液体培养基中以分散的有芽和无芽单细胞生长。在固体培养基上，无毒的变异菌株生长为质地光滑的菌落，而有毒的亲代菌株生长为粗糙的卷曲菌落。在小鼠中进行的毒力测试表明，平滑变体的致死剂量值与无毒的Downs菌株相似，为50％。成对的粗糙和光滑菌株的生长曲线相似。此外，当在一维聚丙烯酰胺凝胶上分离粗细胞级分或通过二维凝胶电泳分离全细胞提取物时，它们具有相同的蛋白质谱。然而，培养物上清液的电泳显示，释放的低分子量肽可能与毒力有关。除了它们在比较毒力研究中的有用性之外，这些无毒力菌株还应被证明对荚膜荚膜梭菌的遗传实验有价值，因为这些酵母菌具有独特的生长能力而不会结块。

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