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首页> 外文期刊>Infection and immunity >Virulence properties and enterotoxin production of Aeromonas strains isolated from fish.
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Virulence properties and enterotoxin production of Aeromonas strains isolated from fish.

机译：从鱼类分离的气单胞菌菌株的毒力特性和肠毒素产生。

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The biological activities in vivo and in vitro of 59 motile Aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. A total of 64.3% of the isolates tested were pathogenic for fish, and 62% of Aeromonas hydrophila and A. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. Although the majority of the strains were proteolytic and amylolytic and produced DNase, other activities, such as elastase and staphylolysis, were only present in A. hydrophila. Most of the strains (96%) produced hemolysins, and 68% had agglutinating capacity, but neither isolates pathogenic for fish nor enterotoxigenic isolates showed specificity for trout or human erythrocytes, respectively. The production of siderophores, agglutination in acriflavine, and precipitation after boiling were found not to be useful tests for screening virulent strains. Although statistical analysis revealed a significant relationship between virulence for fish and positive results for arabinose and sucrose fermentations, elastase, and hemolysis of human erythrocytes, only lysine decarboxylase showed a significant positive relationship with enterotoxigenicity. Using extracellular products from representative Aeromonas strains with different virulence markers and belonging to distinct O serogroups, we demonstrated a lack of correlation between cytotoxicity for fish and homoiothermic cell lines and pathogenicity. The extracellular products from selected pathogenic A. hydrophila strains were lethal for rainbow trout and displayed proteolytic, hemolytic, and cytotoxic activities which were simultaneously lost after heat treatment. The findings reported here indicate that it is not possible to establish a common and single mechanism involved in the invasion of Aeromonas spp. in poikilothermic and homoiothermic hosts.
机译：59种运动型气单胞菌的体内和体外生物活性。从鱼和水箱中分离出的毒素同时在体温和体温系统中进行分析。总共有64.3％的分离株对鱼类具有致病性，而对鱼类有毒或无毒性的嗜水气单胞菌和嗜碱气单胞菌分离株中有62％具有肠毒力。尽管大多数菌株是蛋白水解和淀粉分解的，并产生了DNase，但其他活性，如弹性蛋白酶和葡萄球菌溶解，仅存在于嗜水链球菌中。大多数菌株（96％）产生溶血素，而68％则具有凝集能力，但对鱼类致病的分离株和对产毒素的分离株均未显示出对鳟鱼或人红细胞的特异性。发现铁载体的产生，a啶黄中的凝集和煮沸后的沉淀不是筛选有毒菌株的有用测试。尽管统计分析显示鱼类的毒力与阿拉伯糖和蔗糖发酵，弹性蛋白酶和人类红细胞溶血的阳性结果之间存在显着的相关性，但只有赖氨酸脱羧酶与产肠毒素性呈显着的正相关。使用具有不同毒力标记并属于不同O血清群的代表性气单胞菌菌株的细胞外产物，我们证明了鱼类和同温细胞系的细胞毒性与致病性之间缺乏相关性。选定的致病性嗜水链球菌菌株的细胞外产物对虹鳟鱼具有致死性，并表现出蛋白水解，溶血和细胞毒性活性，这些活性在热处理后会同时丧失。此处报道的发现表明，不可能建立涉及气单胞菌入侵的共同和单一机制。在体温热和等温宿主中。

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