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首页> 外文期刊>Infection and immunity >Relatedness of O-specific lipopolysaccharide side chain genes from strains of Shigella boydii type 12 belonging to two clonal groups and from Escherichia coli O7:K1.
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Relatedness of O-specific lipopolysaccharide side chain genes from strains of Shigella boydii type 12 belonging to two clonal groups and from Escherichia coli O7:K1.

机译：博伊氏志贺氏菌12型菌株（属于两个克隆组）与大肠杆菌O7：K1的O特异性脂多糖侧链基因的相关性。

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摘要

The O-specific lipopolysaccharide side chains of Escherichia coli O7 and Shigella boydii type 12 possess similar but not identical chemical structures. We investigated the genetic relatedness between the O-specific side chain genes in members of these two species. Examination of outer membrane protein and lipopolysaccharide (LPS) banding patterns demonstrated that five strains which had been identified as S. boydii type 12 fell into two clonal groups, SB1 and SB2. Hybridizations with O7-specific radiolabeled probes derived from the chromosomal DNA of an E. coli O7 strain detected identical fragments among the three SB1 strains of S. boydii type 12 and the two E. coli O7 reference isolates. The two other S. boydii type 12 strains, which belonged to the SB2 clone, did not show homologies with the O7 probe under high-stringency conditions of hybridization. The homology between the O7 and type 12 LPS gene regions from the SB1 strains was further confirmed by the construction of O-specific side chain-deficient mutations in these strains by homologous recombination of a suicide plasmid containing O7-specific DNA sequences. Immunoblot experiments with O7 antiserum gave a weak cross-reaction with LPS purified from the SB2 strains but a very strong cross-reaction with the LPS from SB1 isolates. Antiserum raised to one of the SB2 strains cross-reacted only with S. boydii type 12 LPS from the SB1 clone but failed to react with O7 LPS.
机译：大肠杆菌O7和博伊氏志贺氏菌12型的O特异性脂多糖侧链具有相似但不相同的化学结构。我们调查了这两个物种的成员中的O特异性侧链基因之间的遗传相关性。外膜蛋白和脂多糖（LPS）条带模式的检查表明，已被鉴定为12型博伊氏链球菌的5个菌株分为两个克隆组，SB1和SB2。与源自大肠杆菌O7菌株的染色体DNA的O7特异性放射性标记探针杂交，可检测到博伊氏链球菌12型的三个SB1菌株和两个大肠杆菌O7参考菌株中的相同片段。属于SB2克隆的另外两个博伊氏链球菌12型菌株在高严格杂交条件下未与O7探针显示同源性。通过包含O7特异性DNA序列的自杀质粒的同源重组，在这些菌株中构建了O特异性侧链缺陷型突变，进一步证实了来自SB1菌株的O7和12型LPS基因区域之间的同源性。 O7抗血清的免疫印迹实验表明，与从SB2菌株纯化的LPS的交叉反应较弱，但与与SB1分离株的LPS的交叉反应却很强。升至仅与来自SB1克隆的博伊氏链球菌12型LPS交叉反应的SB2菌株之一的抗血清，但未能与O7 LPS反应。

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