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首页> 外文期刊>Infection and immunity >Protective immunity to heartwater (Cowdria ruminantium infection) is acquired after vaccination with in vitro-attenuated rickettsiae.
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Protective immunity to heartwater (Cowdria ruminantium infection) is acquired after vaccination with in vitro-attenuated rickettsiae.

机译：体外减毒立克次体疫苗接种后，可获得对心脏水的保护性免疫（反刍动物反刍动物感染）。

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A Senegalese (S) stock of Cowdria ruminantium was passaged on bovine umbilical endothelial cells with an average interval of 13.9 days (range, 8 to 34 days) between passages. The virulence of infected bovine umbilical endothelial cultures was tested in susceptible goats and sheep by intravenous inoculation of culture supernatant from passages 2 (51 days in vitro), 3 (69 days), 11 (229 days), 14 (264 days), and 16 (291 days). Both animals inoculated with passages 2 and 3 died of heartwater. However, clinical reactions were completely absent in goats and sheep that were inoculated with C. ruminantium from passages 11, 14, and 16. High antibody titers were detected, with immunofluorescence in all vaccinated animals, and a strong signal was found against a 32-kDa Cowdria protein in Western blots (immunoblots). Moreover, the vaccinated animals proved solidly immune when challenged with virulent Cowdria sp.-infected blood stabilate (S strain), whereas all control goats died. No attenuation of a second Cowdria stock (W) was achieved after 226 days in culture, at which time passage 17 was tested in a recipient goat which died of typical heartwater. This is the first report of vaccination with live attenuated C. ruminantium. These attenuated organisms may replace vaccination with virulent blood currently in use in areas where heartwater is endemic.
机译：塞内加尔（S）的考德里亚反刍动物苗在牛脐带内皮细胞上传代，传代之间的平均间隔为13.9天（范围为8到34天）。通过对第2代（体外51天），3（69天），11（229天），14（264天）和3代传代的培养上清液进行静脉接种，在易感山羊和绵羊中测试了感染的牛脐带内皮培养物的毒力16（291天）。接种第2和第3代的两只动物均死于心水。但是，在第11、14和16代接种了反刍动物的山羊和绵羊完全没有临床反应，在所有疫苗接种的动物中都检测到了高抗体滴度和免疫荧光，并且发现了针对32- Western blots中的kDa Cowdria蛋白（免疫印迹）。此外，接种的动物在受到有毒的考德里亚菌感染的血甾醇（S品系）攻击时被证明具有牢固的免疫力，而所有对照山羊均死亡。培养226天后，没有降低第二个Cowdria原种（W），这时在死于典型心水的受体山羊中测试了第17代。这是首次用减毒活小球藻接种疫苗的报告。这些减毒的生物体可以用目前在心脏水很流行的地区使用的强力血液代替疫苗接种。

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