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首页> 外文期刊>Infection and immunity >Passive immunization of Aotus monkeys with human antibodies to the Plasmodium falciparum antigen Pf155/RESA.
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Passive immunization of Aotus monkeys with human antibodies to the Plasmodium falciparum antigen Pf155/RESA.

机译：用抗恶性疟原虫抗原Pf155 / RESA的人抗体对Aotus猴子进行被动免疫。

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In order to assess the protective effects of anti-Pf155/RESA antibodies of different specificities in vivo, passive immunizations of Aotus monkeys were performed. Antibodies reactive with the Pf155/RESA repeat sequences (EENV)2 and EENVEHDA were isolated from the immunoglobulin G (IgG) fraction of a pool of plasmas from Liberia by affinity chromatography on synthetic peptides. The two fractions of antibodies differed in specificity but displayed similar capacities to inhibit merozoite invasion in Plasmodium falciparum in vitro cultures. Four groups of monkeys (named groups I to IV) were injected with (i) 160 mg of total control IgG, (ii) 2 mg of IgG affinity purified on (EENV)2, (iii) 2 mg of IgG affinity purified on EENVEHDA, and (iv) 160 mg of total immune IgG, respectively. The monkeys were then challenged with P. falciparum-infected erythrocytes, and the levels of parasitemia and hematocrits as well as other serological parameters were determined daily. Although all groups developed parasitemia, groups II and IV tended to show lower mean daily levels. Three monkeys of group II and two monkeys (each) of groups III and IV self cured the infections, but so did one monkey from the group treated with control IgG (group I). The serum levels of transfused antibodies were low at the peak of parasitemia, suggesting that clearance of parasites was mediated by immune responses mounted by the monkeys. The results indicate that antibodies to epitopes formed by repeats of Pf155/RESA may depress P. falciparum parasitemias and thus that immunogens based on such repeats should be suitable components in a subunit vaccine against asexual stages of P. falciparum.
机译：为了评估体内不同特异性的抗Pf155 / RESA抗体的保护作用，进行了Aotus猴子的被动免疫。通过合成肽的亲和层析，从利比里亚血浆集合的免疫球蛋白G（IgG）组分中分离出与Pf155 / RESA重复序列（EENV）2和EENVEHDA具有反应性的抗体。这两部分抗体的特异性不同，但是在体外培养中显示出相似的抑制恶性疟原虫入侵裂殖子的能力。向四组猴子（命名为I至IV组）注射（i）160 mg的总对照IgG，（ii）在（EENV）2上纯化的2 mg IgG亲和力，（iii）在EENVEHDA上纯化的2 mg IgG亲和力;和（iv）分别为160毫克的总免疫IgG。然后用恶性疟原虫感染的红细胞攻击猴子，并每天测定寄生虫血症和血细胞比容的水平以及其他血清学参数。尽管所有组均出现寄生虫病，但第二组和第四组倾向于显示较低的平均每日水平。组II的三只猴子和组III和IV的两只猴子（每只）自我治愈感染，但是用对照IgG治疗的组中的一只猴子也治愈了（I组）。在寄生虫血症的高峰期，输血抗体的血清水平较低，这表明寄生虫的清除是由猴子发起的免疫反应介导的。结果表明，针对由Pf155 / RESA重复序列形成的表位的抗体可能会抑制恶性疟原虫寄生虫病，因此，基于此类重复序列的免疫原应该是针对恶性疟原虫无性阶段的亚单位疫苗的合适成分。

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