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首页> 外文期刊>Infection and immunity >HeLa cell adherence, actin aggregation, and invasion by nonenteropathogenic Escherichia coli possessing the eae gene.
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HeLa cell adherence, actin aggregation, and invasion by nonenteropathogenic Escherichia coli possessing the eae gene.

机译：HeLa细胞粘附，肌动蛋白聚集和具有eae基因的非致病性大肠杆菌的侵袭。

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Enteropathogenic Escherichia coli (EPEC) produce diarrhea in humans by a mechanism that involves close adherence to epithelial cells in the intestine and colon. Close adherence is associated with effacement of microvilli and condensation of actin beneath the bacteria, a process termed attaching/effacing adherence. Attaching/effacing adherence of EPEC occurs in vitro in tissue culture, simplifying the study of the molecular genetics of this process. An EPEC gene (eae) necessary for attaching/effacing adherence was recently characterized. Enterohemorrhagic E. coli and the rabbit-specific RDEC-1 strain adhere in a like fashion in vivo and hybridize with eae. However, these strains adhere poorly to tissue culture cells, complicating the in vitro study of attaching/effacing adherence. In order to develop an in vitro model for the study of attaching/effacing activity of non-EPEC bacteria, a plasmid encoding the F1845 adhesin of an E. coli strain (C1845) isolated from a patient with diarrhea was transformed into RDEC-1 and enterohemorrhagic E. coli. The transformed strains adhered in a diffuse pattern to HeLa cells, and they aggregated HeLa cell actin at points of adherence in the fluorescein-isothiocyanate-labeled phalloidin assay. They also invaded HeLa cells in a gentamicin invasion assay, although not to the extent seen with EPEC. The construction of adherent non-EPEC strains facilitates the molecular study of the attaching/effacing properties and invasiveness of these strains in tissue culture models.
机译：肠致病性大肠杆菌（EPEC）通过与肠道和结肠中的上皮细胞紧密粘附的机制在人体内引起腹泻。紧密粘附与微绒毛的清除和细菌下方肌动蛋白的凝结有关，这一过程称为附着/清除粘附。 EPEC的附着/表面附着发生在体外组织培养中，从而简化了此过程的分子遗传学研究。最近表征了附着/消除粘附所必需的EPEC基因（eae）。肠出血性大肠杆菌和兔特异性RDEC-1菌株在体内以相似的方式粘附并与eae杂交。然而，这些菌株对组织培养细胞的粘附性很差，这使附着/表面粘附的体外研究变得复杂。为了开发用于研究非EPEC细菌附着/清除活性的体外模型，将编码自腹泻患者的大肠杆菌（C1845）菌株F1845粘附素的质粒转化为RDEC-1和肠出血性大肠杆菌。在荧光素-异硫氰酸酯标记的鬼笔环肽测定中，转化的菌株以扩散模式粘附在HeLa细胞上，并在粘附点聚集HeLa细胞肌动蛋白。他们还通过庆大霉素入侵试验入侵了HeLa细胞，尽管在EPEC中未见程度。粘附的非EPEC菌株的构建有助于在组织培养模型中对这些菌株的附着/表面特性和侵袭性进行分子研究。

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