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首页> 外文期刊>Infection and immunity >Molecular studies of Ssa1, a serotype-specific antigen of Pasteurella haemolytica A1.
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Molecular studies of Ssa1, a serotype-specific antigen of Pasteurella haemolytica A1.

机译：溶血巴斯德氏菌A1血清型特异性抗原Ssa1的分子研究。

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摘要

A serotype-specific antigen of Pasteurella haemolytica A1 encoded on the recombinant plasmid pSSA1 is characterized. Nucleotide sequence analysis of the insert DNA in pSSA1 identified the gene ssaI, which codes for a protein of approximately 100 kDa. In vivo labeling of pSSA1-encoded protein in Escherichia coli maxicells showed the expression of a 100-kDa protein from the insert DNA on the recombinant plasmid. Northern blot and primer extension analyses were used to identify the mRNA transcript in P. haemolytica A1 and the putative promoter of ssaI. The antigen (designated Ssa1) could be localized to the outer membrane of P. haemolytica A1 and E. coli clones carrying pSSA1. A rabbit serum against Ssa1 was produced by using whole cells of E. coli expressing Ssa1 on the surface as the immunogen, demonstrating that Ssa1 is immunogenic in rabbits. The results from colony immunoblot analysis with calf serum from animals that were resistant to P. haemolytica A1-induced pneumonia suggest indirectly that Ssa1 is also immunogenic in the animals.
机译：表征了重组质粒pSSA1上编码的溶血巴斯德氏菌A1的血清型特异性抗原。 pSSA1中插入DNA的核苷酸序列分析确定了ssaI基因，该基因编码大约100 kDa的蛋白质。大肠杆菌maxicells中pSSA1编码蛋白的体内标记显示重组质粒上插入DNA中有一个100 kDa蛋白的表达。使用RNA印迹和引物延伸分析来鉴定溶血性毕赤酵母A1中的mRNA转录物和ssaI的推定启动子。抗原（命名为Ssa1）可以位于溶血假单胞菌A1和带有pSSA1的大肠杆菌克隆的外膜上。使用表面表达Ssa1的大肠杆菌全细胞作为免疫原，产生了针对Ssa1的兔血清，表明Ssa1在兔中具有免疫原性。用对溶血性疟原虫A1诱导的肺炎具有抗性的动物的小牛血清进行的菌落免疫印迹分析结果间接表明，Ssa1在动物中也具有免疫原性。

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