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首页> 外文期刊>Infection and immunity >Induction of protective immunity by using Anaplasma marginale initial body membranes.
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Induction of protective immunity by using Anaplasma marginale initial body membranes.

机译：通过使用Anaplasma marginale初始体膜诱导保护性免疫。

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Anaplasma marginale initial bodies of the Norton Zimbabwe strain were disrupted and separated into two membrane fractions banding at 1.15 and 1.22 g/cm3 by sucrose density centrifugation. The membrane fractions differed in their morphology and polypeptide composition. Membranes banding at 1.22 g/cm3 shared epitopes with surface-exposed polypeptides of the Florida strain of A. marginale, confirming the outer membrane location of these polypeptides. Immunization of cattle with either membrane fraction induced protection against homologous challenge, as demonstrated by significantly less anemia and lower peak rickettsemia values compared with those of adjuvant-immunized and nonimmunized calves. Protection correlated with antibody titer to membrane polypeptides. Although both membrane fractions induced protection, a 31-kDa polypeptide was the only common antigen to both fractions, as shown by reactivity of immune sera. Identification of membrane antigens capable of inducing protective immunity should facilitate development of vaccines against anaplasmosis suitable for use in Zimbabwe.
机译：通过蔗糖密度离心，将诺顿津巴布韦菌株的无缘无浆膜原始体破坏并分成两个膜部分，分别以1.15和1.22 g / cm3的谱带捆扎。膜级分的形态和多肽组成不同。以1.22 g / cm3的膜带与表皮拟南芥佛罗里达菌株的表面暴露多肽共有表位，确认了这些多肽的外膜位置。与佐剂免疫和未免疫的牛相比，用任一种膜级分对牛进行免疫诱导的针对同源性攻击的保护作用均显示出明显更少的贫血和更低的峰值立克次血值。保护与针对膜多肽的抗体效价相关。尽管两个膜级分均能诱导保护作用，但31-kDa多肽是两个级分的唯一共同抗原，如免疫血清的反应性所示。鉴定能够诱导保护性免疫的膜抗原应有助于开发适用于津巴布韦的抗厌氧菌病疫苗。

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