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首页> 外文期刊>Infection and immunity >Virulence, serotype, and molecular characteristics of environmental strains of Cryptococcus neoformans var. gattii.
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Virulence, serotype, and molecular characteristics of environmental strains of Cryptococcus neoformans var. gattii.

机译：新型隐球菌变种环境菌株的毒力，血清型和分子特征。加蒂。

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Four strains of Cryptococcus neoformans var. gattii originating from Eucalyptus camaldulensis, three from Australia and one from San Francisco, were tested for their serotype, virulence for mice, and a number of genetic and molecular characteristics. All were found to be serotype B and showed significantly higher virulence for mice than did the type strains of C. neoformans var. gattii and Filobasidiella neoformans var. bacillispora, which were obtained from human cryptococcosis cases. Electrophoretic karyotypes of the strains from Australia were identical, although they were collected from sites at least 15 to 500 km apart. The electrophoretic karyotype of the strain from San Francisco was the same as that of the Australian isolates except for the mobility of one chromosome. On the contrary, no two isolates of serotype B (of a total of 11) from clinical sources were the same, regardless of their geographic origin. Furthermore, none of the clinical isolates showed a chromosomal banding pattern identical to that of Eucalyptus-originated strains. The Eucalyptus-originated strains failed to form dikaryons when crossed with the tester strains of the two varieties of F. neoformans. Hybridization analysis with a nucleic acid probe (AccuProbe C. neoformans Culture Confirmation Test; Gen-Probe Inc., San Diego, Calif.), however, showed signals of equal intensity for clinical strains and the Eucalyptus-originated strains. Various fungi phylogenetically related to C. neoformans, including a phenol oxidase-positive strain of Cryptococcus laurentii obtained from E. camaldulensis, were negative in the nucleic acid hybridization test. These observations confirm that, in spite of karyotypic differences and the lack of dikaryon formation with the tester strains of F. neoformans, Eucalyptus-originated C. neoformans var. gattii is the same organism as those isolated from cases of human infection. Furthermore, the C. neoformans culture confirmation test using a commercial nucleic acid probe is specific for C. neoformans.
机译：四株新隐球菌变种。测试了源自澳大利亚桉树的加蒂，来自澳大利亚的三只和来自旧金山的一头加蒂的血清型，对小鼠的毒力以及许多遗传和分子特征。发现它们全部都是血清型B，并且对小鼠显示出比新孢梭菌变种株显着更高的毒力。加蒂和新形成丝藻芽孢杆菌，是从人类隐球菌病病例获得的。来自澳大利亚的菌株的电泳核型相同，尽管它们是从相距至少15至500 km的站点收集的。来自旧金山的菌株的电泳核型与澳大利亚分离株的电泳核型相同，只是一条染色体具有迁移性。相反，无论来自何处，从临床来源分离出的两种B型血清型（总共11种）都是相同的。此外，没有临床分离株显示出与桉树起源菌株相同的染色体谱带模式。源自桉树的菌株在与新福尔摩斯氏菌两个变种的测试菌株杂交时未能形成双核。但是，用核酸探针（AccuProbe C. neoformans培养确认测试; Gen-Probe Inc.，加利福尼亚州圣地亚哥）进行的杂交分析显示，临床菌株和桉树菌株的信号强度相同。在系统杂交方面，与新甲隐球菌相关的各种真菌，包括从卡氏大肠杆菌中获得的劳伦隐球菌的酚氧化酶阳性菌株，在核酸杂交试验中均为阴性。这些观察结果证实，尽管有核型差异和新福尔摩斯氏菌的测试菌株缺乏双核体形成，但桉树起源的新福尔摩斯氏菌仍存在。加蒂（Gattii）与从人类感染病例中分离出的生物是同一生物。此外，使用市售核酸探针的新孢子虫培养物确认试验对新孢子虫具有特异性。

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